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Keywords
Molecular Evolution
Molecular Evolution
Publications: 4,501| Citation Count: 106,796
Stemming Variations: molecular evolut, Molecular evolutional, Molecular evolutions
Cumulative Annual
    • Molecular evolution is a powerful means of engineering proteins. It usually requires the generation of a large recombinant DNA library of variants for cloning into a phage or plasmid vector, and the transformation of a host organism for expression and screening of the variant proteins. However, library size is often limited by the low yields of circular DNA and the poor transformation efficiencies of linear DNA...

    Daniel Christ, et al. Tapping diversity lost in transformations--in vitro amplification of l...

    • Molecular evolution is a powerful tool for exploring and creating catalytic function in biological macromolecules. This review focuses on examples and current strategies for the application of random mutagenesis of target genes coupled with direct selection in vivo. The technology is illustrated by the dissection of catalytic features in chorismate mutase...

    Peter Kast, et al. Genetic selection strategies for generating and characterizing catalys...

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