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Keywords
Locked Nucleic Acid

LNA,Locked Nucleic Acid,Locked Nucleic Acids

Locked Nucleic Acid - LNA
Publications: 486| Citation Count: 6,252
Stemming Variations: Locked Nucleic Acids
Cumulative Annual
    • Locked Nucleic Acid (LNA) is a novel, third generation DNA analogue that has the potentialto impact strongly on the future development of a diversity of nucleic acid based technologies.The present chapter reviews the known biochemical properties of LNA and exemplifies how thesehave been used to improve both DNA diagnostic technologies and antisense therapeutics...

    Henrik Ørumet al. Locked nucleic acids (LNA) and medical applications

    • Locked nucleic acid (LNA) is a nucleic acid analog containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA-mimicking sugar conformation. This conformational restriction is translated into unprecedented hybridization affinity towards complementary single-stranded RNA molecules...

    S. Kauppinenet al. Locked Nucleic Acid: High-Affinity Targeting of Complementary RNA for ...

    • Locked nucleic acid (LNA ® ) is a modified DNA with increased binding affinity for complementary DNA sequences. Our strategy was to use this property of LNA to inhibit undesired PCR amplification (e.g., from contaminating genomic DNA) in a cDNA-based assay. By placing a short complementary LNA sequence in intronic DNA, the aim was to inhibit the amplification of genomic DNA without affecting the amplification of reverse-transcribed spliced mRNA. LNA was designed to bind within intron 5 in the x-box binding protein 1 (XBP1) gene. An irrelevant LNA oligonucleotide served as a negative control...

    Lone Hummelshojet al. Locked nucleic acid inhibits amplification of contaminating DNA in rea...

    • Locked nucleic acid (LNA) is a deoxyribonucleotide analogue with an unusual ‘locked’ furanose conformation. LNA-modified oligonucleotide probes have demonstrated an enhanced binding affinity towards their complementary strands; however, their potential to discriminate non-complementary hybridization of mismatches has not been explored...

    XianYu Piaoet al. Enhanced recognition of non-complementary hybridization by single-LNA-...

    • Locked nucleic acid (LNA) is a chemically modified nucleic acid with its sugar ring locked in an RNAlike (C3'-endo) conformation. LNAs show extraordinary thermal stabilities when hybridized with DNA, RNA or LNA itself...

    Vineet Pandeet al. Insights into structure, dynamics and hydration of locked nucleic acid...

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