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Elucidation of the structure of cellulolytic enzyme lignin

Elucidation of the structure of cellulolytic enzyme lignin,10.1515/HF.2006.061,Holzforschung,Zhoujian Hu,Ting-Feng Yeh,Hou-min Chang,Yuji Matsumoto,Jo

Elucidation of the structure of cellulolytic enzyme lignin   (Citations: 17)
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Cellulolytic enzyme lignin (CEL) and milled wood lignin (MWL) were prepared by three different ball-milling meth- ods. The structure of CEL at various yields was eluci- dated and compared with MWL using wet chemical analysis, FTIR and solution-state NMR techniques. Results show that ball milling of wood degrades b-O-4 structures in lignin. However, even after extensive ball milling, less than 25% of the b-O-4 structures were degraded. The extent of degradation was less for soft- wood than for hardwood lignin. Extractable lignin yield, either MWL or CEL, was the best way to assess the extent and effect of ball milling. CEL is preferred over MWL, as it can be isolated in higher yield with less deg- radation. CEL was isolated at yields ranging from 20% to 86%. Over this range the CEL had similar structures, suggesting that lignin in the secondary wall is uniform in structure. The residual enzyme lignin (REL) was structur- ally different from CEL and may originate mainly from the middle lamella. In this paper we propose a new proce- dure for the isolation of lignin for use in structural studies, whereby wood is sufficiently milled and successively extracted to produce three lignin fractions representing the total lignin in wood.
Journal: Holzforschung , vol. 60, no. 4, pp. 389-397, 2006
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    • ...It is well know that they are cleaved in certain extend during ball milling (Fujimoto et al. 2005; Hu et al. 2006)...

    Mikhail Balakshinet al. Quantification of lignin–carbohydrate linkages with high-resolution NM...

    • ...An expanded approach was subjected to milled wood to enzymatic treatment to remove most of the carbohydrate components and followed by a liquid-solid extraction (8,9)...

    Xueming Zhanget al. Separation and Structural Characterization of Lignin from Hybrid Popla...

    • ...Other approaches are: 1) subjecting the milled wood to enzymatic treatment with cellulolytic enzymes to remove most of the carbohydrate components (Chang et al. 1975; Wu and Argyropoulos 2003; Holtman et al. 2004; Hu et al. 2006); 2) completely dissolving ball-milled wood in a solvent system (dimethylsulfoxide, DMSO, and N-methylimidazol, NMI) followed by precipitation in dioxane/water in the course of which lignin and carbohydrate ...
    • ...Indeed, it has also been demonstrated that MWL can undergo some structural modifications during isolation, especially during the milling process (Fujimoto et al. 2005; Guerra et al. 2006; Hu et al. 2006; Balakshin et al. 2008)...

    Jorge Rencoretet al. HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea ab...

    • ...In addition, based on the increase in α-aryl ether groups, it was suggested that some condensation reactions may occur during the ball-milling process (10)...

    Feng Xuet al. Fractional Separation of Hemicelluloses and Lignin in High Yield and P...

    • ...However, concerns exist over the similarity between MWL and native lignin based on the low yields (25‐50% of protolignin) and structural alterations due to ball milling (Hu et al. 2006), since ball milling reduces the degree of polymerization, creating new free phenolic hydroxyl groups through cleavage of b-aryl ether linkages and increasing a-carbonyl groups via sidechain oxidation (Fujimoto et al. 2005)...
    • ...It is well known that lignin in the primary wall (P), lignin in the secondary wall (S2) and lignin in the compound middle lamella (CML) have different chemical structures (Hu et al. 2006; Yang and Goring 1980; Whiting and Goring 1982)...

    Feng Xuet al. Fractional isolation and structural characterization of mild ball-mill...

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