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The species-specific egg receptor for sea urchin sperm adhesion is EBR1,a novel ADAMTS protein

The species-specific egg receptor for sea urchin sperm adhesion is EBR1,a novel ADAMTS protein,10.1101/gad.1133003,Genes & Development,Noriko Kamei,Ch

The species-specific egg receptor for sea urchin sperm adhesion is EBR1,a novel ADAMTS protein   (Citations: 47)
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Species-specific adhesion of sperm to the egg during sea urchin fertilization involves the interaction of the sperm adhesive protein,bindin,and a complementary receptor on the egg surface,and serves to restrict the gene pool to individuals of the same species. We used PCR represen- tation difference analysis to clone the species-specific egg receptor for bindin,EBR1,from Strongylocentrotus franciscanus (Sf) and S. purpuratus (Sp). Sf-EBR1 con- tains a novel ADAMTS-like N-terminal domain fol- lowed by ∼19 tandem EBR repeats consisting of alternat- ing CUB and thrombospondin type 1 (TSP-1) domains where the last 10 EBR repeats are species-specific and highly conserved. Recombinant protein corresponding to the species-specific EBR repeat displays species-specific sperm adhesion and bindin-binding activity. The Sp- EBR1 ortholog has the same ADAMTS (a disintegrin and metalloprotease with thrombospondin type-1 modules) core region followed by eight and one-half tandem egg bindin receptor (EBR) repeats that share 88% identity with the Sf-EBR1 repeats,but has an entirely different species-specific domain consisting of hyalin-like (HYR) repeats. Thus,the species-specific domains of egg bindin receptor 1 (EBR1) from both species function as the egg surface receptor to mediate species-specific sperm adhe- sion.
Journal: Genes & Development - GENE DEVELOP , vol. 17, no. 20, pp. 2502-2507, 2003
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