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Secondary metabolites synthesis in transformed cells of Glycyrrhiza glabra L. and Potentilla alba L. as producents of radioprotective compounds

Secondary metabolites synthesis in transformed cells of Glycyrrhiza glabra L. and Potentilla alba L. as producents of radioprotective compounds,P. G.

Secondary metabolites synthesis in transformed cells of Glycyrrhiza glabra L. and Potentilla alba L. as producents of radioprotective compounds   (Citations: 1)
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Following the Chernobyl explosion, screening for new drugs of radioprotective activity has been initiat� ed in Ukraine. The European licorice (Glycyrrhiza glabra L.,) is a pharmacologically important species which is rich in flavonoids and saponins, especially in the roots. To increase the pharmacological potential of this plant, the authors have obtained transformed licorice protoplasts with higher production of target compounds. Isolated licorice G.glabra L. protoplast suspension was electroporated by the chimeric plasmid pDNt23�CaMV35SnosnptIIcat (pDNt23� rootspecific and CaMV 35S promoters, nosnopaline synthase terminator, and selectable NPTII gene). On the selec� tive medium with addition of kanamycine sulphate, proorganogenic clusters have been obtained. These obtained cell clusters have been additionly inoculated by Agrobacterim rhizogenes (15834). In general, the level of secondary metabolites in these transformed cells was 2�fold higher than in nontransformed control cells. We found that flavonoids isolated from transformed G.glabra cells possess aldose reductase inhibitory activities; these compounds have been tested on the rat lens. The intact root segments of Potentilla alba L. have been also inoculated by this strain too, to obtain «hairy roots» as terpens producent. These compounds have shown inhibitory effect on the thyroxine lev� els in white rat thyroid glands. These results suggested that metabolites obtained from transformed pharmaceutical plant species can be used as radioprotective compounds after γ�irradiation.
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