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Multicenter Evaluation of the Updated and Extended API (RAPID) Coryne Database 2.0

Multicenter Evaluation of the Updated and Extended API (RAPID) Coryne Database 2.0,GUIDO FUNKE,FRANCOIS N. R. RENAUD,JEAN FRENEY,PHILIPPE RIEGEL

Multicenter Evaluation of the Updated and Extended API (RAPID) Coryne Database 2.0   (Citations: 38)
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In a multicenter study, 407 strains of coryneform bacteria were tested with the updated and extended API (RAPID) Coryne system with database 2.0 (bioMerieux, La-Balme-les-Grottes, France) in order to evaluate the system's capability of identifying these bacteria. The design of the system was exactly the same as for the previous API (RAPID) Coryne strip with database 1.0, i.e., the 20 biochemical reactions covered were identical, but database 2.0 included both more taxa and additional differential tests. Three hundred ninety strains tested belonged to the 49 taxa covered by database 2.0, and 17 strains belonged to taxa not covered. Overall, the system correctly identified 90.5% of the strains belonging to taxa included, with additional tests needed for correct identification for 55.1% of all strains tested. Only 5.6% of all strains were not identified, and 3.8% were misidentified. Identification problems were observed in particular for Corynebacterium coyleae, Propionibacte- rium acnes, and Aureobacterium spp. The numerical profiles and corresponding identification results for the taxa not covered by the new database 2.0 were also given. In comparison to the results from published previous evaluations of the API (RAPID) Coryne database 1.0, more additional tests had to be performed with version 2.0 in order to completely identify the strains. This was the result of current changes in taxonomy and to provide for organisms described since the appearance of version 1.0. We conclude that the new API (RAPID) Coryne system 2.0 is a useful tool for identifying the diverse group of coryneform bacteria encountered in the routine clinical laboratory. It is common knowledge that the identification of coryne- form bacteria is one of the most difficult tasks for clinical bacteriologists. This is mainly due to the enormous diversity of these organisms and the small number of readily available conventional tests that can be used to differentiate them. The API (RAPID) Coryne system (bioMerieux, La-Balme-les- Grottes, France), introduced in the early 1990s (1, 7, 11), was the first specific commercial identification system for coryne- form bacteria and has since proved itself invaluable to many clinical laboratories. Because our knowledge of the diversity of coryneform bacteria encountered in clinical specimens has dra- matically increased since the early 1990s (6), it has become appropriate to broaden and adopt the API Coryne (RAPID) database, considering both changes in taxonomy and newly described taxa. (The term API Coryne instead of API (RAP- ID) Coryne will be used from here on throughout the whole article.) Version 1.0 of the API Coryne database contained only 33 taxa, whereas the new version 2.0 covers 49 taxa. A multicenter study was created to evaluate this most recent database. The design of the study was such that numerical distribution of the strains tested did not reflect their frequency of isolation in clinical specimens but was rather directed to challenging the depth of the new database with a heteroge- neous group of organisms. It is finally important to note that the design of the API Coryne strip was not altered by the company (i.e., the same 20 biochemical reactions were still included) but that the new database contained many more additional easy-to-perform tests useful in the differentiation of coryneform bacteria.
Published in 1997.
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