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Method for the Determination of Vitamin E (Total Tocopherols) in Serum

Method for the Determination of Vitamin E (Total Tocopherols) in Serum,Robert G. Martinek

Method for the Determination of Vitamin E (Total Tocopherols) in Serum   (Citations: 26)
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The useof tripyridyltriazine hasbeeninvestigated asa chromogenic reagentfor the colorimetric determination of vitaminE in serum. The advantages of usingthis re- agenthavebeenelucidated.Comparison ismadewith a methodemploying terpyridyl. A.CONSIDERABLE LITERATURE has developed with respect to the deter- mination of vitamin E as total tocopherols. Several texts (1-3) pre- sent both chemical and biologic assay methods. Although the direct spectrophotometric determination of vitamin E in serum at its absorp- tion peak of about 295 m is attractive, it is not practical because of very low sensitivity and the presence of many interfering substances. Only two of the oxidimetric color reactions for vitamin E appear sen- sitive enough for analysis of serum. One is oxidation (e.g., with silver nitrate) to give tocopheryiquinone with an absorption peak at about 265 m; it requires rigid control of conditions for precise results. The other is the Emmerie and Engel (4) color reaction with ferric chloride and , '-dipyridyl (bipyridyl) to give a red color, a reaction that is precise and easy to perform, and therefore the approach of choice for
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