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Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct

Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct,10.1590/S1516-89132004000500018,Brazilian Archives

Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct   (Citations: 10)
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Pectin lyase and polygalacturonase production by newly isolated fungal strains was carried out in solid-state fermentation. Moniliella SB9 and Penicillium sp EGC5 produced polygalcturonase (PG) and pectin lyase (PL) on mixture of orange bagasse, sugar cane bagasse and wheat bran as substrate. PG and PL produced by Moniliella presented optimum activity at pH 4.5 and 10.0 and at 55 and 45°C, respectively, while these enzymes from Penicillium sp presented optimum activity at pH 4.5-5.0 and 9.0 and 40°C, respectively.
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    • ... moniliforme Wheat bran and orange pulp 81.0 (18) Penicillium viridicatum Orange bagasse and wheat bran 71.2 (35) Thermoascus auriantacus Wheat bran or orange bagasse 43.0 (22) Aspergillus niger Sugar cane bagasse and pectin 38.0 (13) Aspergillus niger Wheat bran and dextrose 36.3 (36) Lentinus edodes Strawberry pomace 29.4 (37) Aspergillus niger Wheat bran and pectin 27.5 (38) Moniliella sp. Sugar cane bagasse and wheat bran 26.0 (39) ...
    • ... bran or orange bagasse 43.0 (22) Aspergillus niger Sugar cane bagasse and pectin 38.0 (13) Aspergillus niger Wheat bran and dextrose 36.3 (36) Lentinus edodes Strawberry pomace 29.4 (37) Aspergillus niger Wheat bran and pectin 27.5 (38) Moniliella sp. Sugar cane bagasse and wheat bran 26.0 (39) Aspergillus awamori Grape pomace 25.0 (40) Chaetomium sp. Palm oil mill fibre 18.9 (41) Penicillium sp. Sugar cane bagasse and wheat bran 12.0 (39) ...

    Ernesto Favela-Torreset al. Production of Hydrolytic Depolymerising Pectinases

    • ...electrophoresis (SDS-PAGE), using 3% and 10% acrylamide concentrations for the stacking and running gels, respectively, each containing 0.1% SDS (Laemmli 1970)...
    • ...distilled water several times (Laemmli 1970)...

    Nazan Demiret al. Purification and characterization of a pectin lyase produced by Geobac...

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