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Size fractionation of double-stranded DNA by precipitation with polyethylene glycol

Size fractionation of double-stranded DNA by precipitation with polyethylene glycol,10.1093/nar/2.3.383,Nucleic Acids Research,John T. Lis,Robert Schl

Size fractionation of double-stranded DNA by precipitation with polyethylene glycol   (Citations: 27)
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We show that DNA molecules of differing molecular mass are separable by selective precipitation with polyethylene glycol (PEG*). Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA. Double-stranded DNA can be fractionated at least in the range of 3 X 10? to 1 X ICK daltons. The effects of PEG concentration, sodium chloride concentration, DNA concentration, pH, divalent ions, precipitation time, and centrifugal force have been determined. These studies shov; PEG precipitation offers a size fractionation method for DNA which is convenient, of high capa- city, and applicable over a wide range of conditions. However, resolution is not high and separation of two species approaches 100$ only if they differ in molecular mass by at least a factor of two.
Journal: Nucleic Acids Research - NAR , vol. 2, no. 3, pp. 383-390, 1975
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