Determination of D-Xylose in Urine for the D-Xylose Absorption Test

Determination of D-Xylose in Urine for the D-Xylose Absorption Test,Alvin L. Harris

Determination of D-Xylose in Urine for the D-Xylose Absorption Test   (Citations: 5)
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A method is describedfor the determination of D-xylOSein urine which is basedon the specificabsorptivityof the pentoseo-toluidinecomplex.Quantitationof xylose can be madein the presence of a hexose. HE DETERMINATION OF XYLOSE in both blood amid urine has been em- ployed in the absorption test for the clinical evaluation of patients with celiac disease, steatorrhea, and nontropical sprue (1-5). The first meth- od for the xylose absorption test was introduced by Helmer and Foutes in 1937 (6) and employed the Folin procedure for glucose. Subsequently, other methods utilizingreactions with bromo-anihine (7, 8) and orcinol (9, 10), and the Somogyi-Nelson procedure (1), as well as comparisons of all these methods (1, 2, 7, 8), have been reported. The relation be- tween the xylose dose given tile patient, and the method of analysis and its correlation with clinical findings also have been discussed (3). All of the foregoing methods share one or more of the common dis- advantages usually encountered in xylose determination-e.g., the non- specificity of reaction for xylose, interference by glucose or other hexoses, elaborate procedural steps, special organic or caustic solvents, or a combination of any or all of these. In the procedure described here, an o-toluidine reagent is used to determine n-xylose in urine. This reagent is in wide general use for the determination of blood and urine glucose, as introduced by Hultmaii (ii). It is not specific for glucose and has a greater sensitivity for xylose.
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