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Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114

Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114,10.1093/nar/gkl1162,Nucleic Acids Research,Hiroyuki Sasanuma,Hajime Murakami,

Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114   (Citations: 14)
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Meiotic recombination is initiated by DNA double- stranded break (DSB) formation catalyzed by Spo11, a type-II topoisomerase-like transesterificase, presumably via a dimerization-mediated mech- anism. We demonstrate the existence of in vivo interactions between Spo11 proteins carrying distinct tags, and the chromatin-binding and DSB activity of tagged Spo11at innate and targeted DSB sites upon fusion to the Gal4 DNA-binding domain. First we identified the interaction between Spo11- 3FLAG and Gal4BD-Spo11 proteins, and established that this interaction specifically occurs at the time of DSB formation. We then observed that presence of the Gal4BD-spo11Y135F (nuclease-deficient) protein allows Spo11-3FLAG recruitment at the GAL2 locus, indicative of the formation of a hetero-complex near the GAL2 UAS sites, but no formation of double- or single-strand breaks. Spo11 self-interaction around the GAL2 DSB site depends on other proteins for DSB formation, in particular Rec102, Rec104 and Rec114. Together, these results suggest that in vivo self-association of Spo11 during meiosis is genetically regulated. The results are discussed in relation to possible roles of Spo11 self-interaction in the control of the cleavage activity.
Journal: Nucleic Acids Research - NAR , vol. 35, no. 4, pp. 1119-1133, 2007
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    • ...In addition, Spo11 association with chromatin requires Rec102/Rec104 (Prieler et al. 2005; Sasanuma et al. 2007)...
    • ...Rec114 localizes independently of other DSB proteins to the DNA and is needed for Spo11 association with hot-spot DNA (Prieler et al. 2005; Sasanuma et al. 2007; Maleki et al. 2007)...

    Silvia Steineret al. Functional interactions among members of the meiotic initiation comple...

    • ... preferentially binds to centromeres (centromeres in budding yeast induce high cohesin binding spanning several kilobases of DNA, Weber et al. 2004; Eckert et al. 2007; Kogut et al. 2009) and other Rec8 binding sites in early meiosis, sites that are characteristically devoid of meiotic DNA DSBs and/or recombination (e.g., Lambie and Roeder 1986, 1988; Gerton et al. 2000; Blat et al. 2002; Glynn et al. 2004; Mieczkowski et al. 2006; Sasanuma et ...

    Amit Bardhan. Many functions of the meiotic cohesin

    • ...Moreover, the formation of the meiosis-specific Spo11 multimer, which is essential to DSBs and requires Rec102 and Rec104 functions, has not been detected in the domains (Sasanuma et al., 2007; Fukuda et al., 2008)...

    Kazuto Kugouet al. Rec8 Guides Canonical Spo11 Distribution along Yeast Meiotic Chromosom...

    • ...The findings in this study reinforce the view that has emerged from several lines of inquiry that there are at least four functionally distinct subgroups among the DSB proteins: Spo11–Ski8; Rec102–Rec104; Mer2–Mei4–Rec114; and Mre11–Rad50–Xrs2 (see the “Introduction”) (Ohta et al. 1998 ;J iao et al.2003; Arora et al. 2004; Kee et al. 2004; Prieler et al. 2005; Henderson et al. 2006 ;L i et al.2006; Sasanuma et al. 2007)...
    • ... another: they interact physically and genetically, they are mutually interdependent for proper chromosome localization, their behaviors are affected (or not) in parallel ways by mutations in other genes, and absence of either one exerts the same kind of effect (or not) on the behavior of other proteins (Salem et al. 1999; Jiao et al. 2003; Arora et al. 2004; Kee et al. 2004; Prieler et al. 2005; Henderson et al. 2006; Li et al. 2006; Sasanuma ...
    • ...assessed by chromatin immunoprecipitation (Prieler et al. 2005) and for the ability of Gal4BD–Spo11 to recruit other Spo11 molecules to a Gal4 binding site on chromatin (Sasanuma et al. 2007)...
    • ...Physical and functional connections of Rec102–Rec104 with Spo11 are well established (Salem et al. 1999; Kee and Keeney 2002; Jiao et al. 2003; Arora et al. 2004; Kee et al. 2004; Prieler et al. 2005; Sasanuma et al. 2007)...
    • ...Moreover, this hypothesis is consistent with the observation that both Rec114 and the Rec102– Rec104 complex are required for binding of Spo11 to hotspots (Prieler et al. 2005; Sasanuma et al. 2007)...

    Shohreh Malekiet al. Interactions between Mei4, Rec114, and other proteins required for mei...

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