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Simple Method for Determination of Thiocyanate in Urine

Simple Method for Determination of Thiocyanate in Urine,M. Rezaul Haque,J. Howard Bradbury

Simple Method for Determination of Thiocyanate in Urine   (Citations: 13)
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Background: It would be useful to develop a simple kit method for determination of thiocyanate in urine, which could be used to monitor cyanide overload in cassava- consuming populations. Methods: The method was based on the quantitative oxidation of thiocyanate in acid permanganate at room temperature in a closed vial with liberation of HCN, which reacted with a picrate paper. For semiquantitative analysis in the field, the colored picrate paper was matched with a color chart prepared using known amounts of KSCN. In the laboratory, a more accurate result was obtained by elution of the colored complex in water and measurement of the absorbance at 510 nm. Over the range 0 -100 mg/L, there was a linear relation- ship given by the equation: thiocyanate content (mg/ L) 5 78 3 absorbance. Results: The picrate thiocyanate method gave no inter- ference with urine samples containing protein at less than 7 g/L, 21 amino acids, histamine, glucose, NaCl, urea, blood, and linamarin. For 53 urine samples ana- lyzed by an accurate column method and the thiocya- nate picrate method, a regression line gave very good agreement (r 2 5 1.000). Quantitative recoveries of thio- cyanate added to urine samples were obtained with the picrate method. Conclusions: A simple picrate kit for determination of thiocyanate in urine was developed and is available free of charge for workers in developing countries. © 1999 American Association for Clinical Chemistry
Published in 1999.
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    • ...Methods of analysis include spectrophotometry (37, 59, 62, 74, 78, 81, 89, 90, 93, 105, 120, 130, 133, 139, 141, 143, 145,146,147,148,149,150,151,152,153,154,155,156,157,158,159,160,161,162,163,164,165,166,167,168,169,170,171,172,173,174), fluorescence (49, 51, 89, 115, 121, 132, 142, 175,176,177,178,179,180,181), chemiluminescence (77, 109), electrochemistry (18, 56, 63,64,65, 68, 69, 73, 75, 79, 80, 106, 129, 138, 153, 182,183,184,185,186,187,188,189,190,191,192,193,194,196, 197), gas chromatography (GC) (45, 53, 54, 61, 91, 95, 103,104,105, 107, 108, 111, 113, 114, 117, 119, 121, 125, 126, 128, 131, 135, 137, 152, 198,199,201,202,203,204,205,206,207,208,209,210,211,212), liquid chromatography (LC) (34, 40, 49, 52, 66, 67, 72, 92, 94, 109, 110, 112, 115, 122, 134, 142, 153, 162, 190,191,192, 213,213,215,216,217,218,219,220,221,222,223,224,225,226,227,228), flow injection analysis (FIA) (64, 109, 191, 193, 215), capillary electrophoresis (CE) (70, 229, 230), and atomic absorption (AA) (231, 232)...

    Brian A. Logueet al. The Analysis of Cyanide and its Breakdown Products in Biological Sampl...

    • ...(37) ppm (range10–200 ppm) was obtained from 80 cassava flour samples from Nampula Province of Mozambique in a year of normal rainfall.33 The...

    Dulce Nhassicoet al. Rising African cassava production, diseases due to high cyanide intake...

    • ...Determination of urinary thiocyanate measures the recent intake of cyanide (Carlsson, Mlingi, Juma, Ronquist, & Rosling, 1999); hence another picrate kit was developed to measure urinary thiocyanate (Haque, & Bradbury, 1999)...

    M. Rezaul Haqueet al. Preparation of linamarin from cassava leaves for use in a cassava cyan...

    • ...4 5 6 7 8 9 10 11 12 The conventional Fe(III)–SCN was reported for spectrophotometric determination of thiocyanate but the method is less sensitive...

    Aditya Shuklaet al. Determination of Thiocyanate in Waste Water

    • ...These measurements have been greatly expedited by the recent development of simple kits (Egan et al., 1998; Bradbury et al., 1999; Haque and Bradbury, 1999)...
    • ...Other objectives were to detect subclinical upper motor neuron damage, as manifested by ankle clonus, in schoolchildren and to test a new picrate kit for measuring urinary thiocyanate concentration (Haque and Bradbury, 1999)...
    • ...Urine samples were stored at room temperature for up to 30 h and refrigerated at 2– 4 ° Cf or up to 24 h before analysis and the thiocyanate content was stable under these conditions (Haque and Bradbury, 1999)...
    • ...Single analyses of thiocyanate in urine samples were made using a new picrate kit method and the thiocyanate content (mol l −1) obtained by multiplying the thiocyanate content in ppm by 17.2 (Haque and Bradbury, 1999)...

    Mario Ernestoet al. Persistent konzo and cyanogen toxicity from cassava in northern Mozamb...

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