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Why is transcription coupled to translation in bacteria?

Why is transcription coupled to translation in bacteria?,J. Gowrishankar,R. Harinarayanan

Why is transcription coupled to translation in bacteria?   (Citations: 28)
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Summary Active mechanisms exist to prevent transcription that is uncoupled from translation in the protein-coding genes of bacteria, as exemplified by the phenomenon of nonsense polarity. Bacterial transcription-transla- tion coupling may be viewed as one among several co-transcriptional processes, including those for mRNA processing and export in the eukaryotes, that operate in the various life forms to render the nascent transcript unavailable for formation of otherwise del- eterious R-loops in the genome. The lack of a membrane-enclosed nucleus, a classical feature that distinguishes a prokaryote from a eukaryote, explains how transcription may be coupled to translation in the former but does not explain why this should happen. That such coupling is not an incidental consequence of the absence of a spatial barrier is underscored by the phenomenon of nonsense polarity in bacteria, first identi- fied in the lac and trp operons of Escherichia coli nearly 40 years ago (Newton et al ., 1965; Yanofsky and Ito, 1965). Nonsense polarity refers to the abolition of expres- sion of intact promoter-distal genes in an operon that bears a nonsense mutation which stops translation in a promoter-proximal gene, and is mediated by premature termination of transcripts in the region immediately down- stream of the nonsense mutation (Adhya and Gottesman, 1978; Nudler and Gottesman, 2002). The common thinking is that transcription-translation coupling is a means for the cell to prevent accumulation of non-functional transcripts in the cytoplasm (Richard- son, 1991; 2002), and hence that it is functionally analo- gous to nonsense-mediated mRNA decay that occurs in
Published in 2004.
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    • ... However, the nascent RNA has the potential to anneal back to the transcribed strand, creating a stable RNA–DNA hybrid and leaving the NTS exposed as an extended single strand of DN...

    Nayun Kimet al. Transcription as a source of genome instability

    • ...While it is true for eukaryotes that the multi-level organization of gene regulation obfuscates the connection between mRNA and protein levels, let alone metabolic fluxes, and it seems that most of the control on metabolism is contributed by the post-transcriptional levels [22], the situation is known to be quite different in prokaryotes where transcription and translation are tightly coupled [23,24]...
    • ...Flux balance analysis (FBA) is a quantitative approach for computing steady-state fluxes on metabolic networks [23]...

    Nikolaus Sonnenscheinet al. Analog regulation of metabolic demand

    • ...was required to diffuse to the membrane before translation could occur; Equation 6 was limited to membrane sites. Since ribosomes likely attach to an mRNA's RBS while transcription is still ongoing ...

    Elijah Robertset al. Noise Contributions in an Inducible Genetic Switch: A Whole-Cell Simul...

    • ...Furthermore and since kanamycin blocks translation and in bacteria the transcription is coupled to translation (Gowrishankar and Harinarayanan 2004), we have also checked if there was any influence of the antibiotic used in the number of read-through transcripts...
    • ...Even though in bacteria transcription is coupled to translation (Gowrishankar and Harinarayanan 2004), it seems unlikely that RNA–DNA hybrids could permit a ribosome assembly and subsequent aph translation...

    Sofia C. Ribeiroet al. Evidence for the in vivo expression of a distant downstream gene under...

    • ... In prokaryotes, ribosomes bind to nascent mRNAs so that translation can be synchronous with transcription; proteins levels thus depend more directly on mRNA abundance ...

    Caroline Colijnet al. Interpreting Expression Data with Metabolic Flux Models: Predicting My...

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