Academic
Publications
OPTIMALISASI EKSTRAKSI DNA DAN PCR-RAPD PADA Grevillea spp. (PROTEACEAE)

OPTIMALISASI EKSTRAKSI DNA DAN PCR-RAPD PADA Grevillea spp. (PROTEACEAE),MADE PHARMAWATI

OPTIMALISASI EKSTRAKSI DNA DAN PCR-RAPD PADA Grevillea spp. (PROTEACEAE)  
BibTex | RIS | RefWorks Download
Molecular genetic analysis of plants relies on high yield and high purity of DNA as well as optimized condition of molecular reactions. Appropriate methods for DNA extraction and molecular reactions such as PCR are therefore needed. This study aimed to develop protocol for extraction of high molecular weight DNA from Grevillea leaf and to optimize condition of PCR-RAPD. Standard plant DNA extraction of Doyle and Doyle was modified by increasing EDTA concentration to 50 mM and addition of 2% (v/v) 2-mercaptoethanol. Moreover, incubation time was prolonged to �4-�6 h at 55 o C. This method yielded good quality of DNA and consistent results. Amplification of DNA using PCR-RAPD will become efficient and consistent if the amplification reactions are in ideal condition. In Grevillea, clear, reproducible and scorable PCR-RAPD patterns were obtained using �0ng DNA template, 5 pmol primer, 2.5 mM MgCl2 and the number of thermal cycle was 40 x.
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.