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Secretory Immune Response to Membrane Antigens during Giardia lamblia Infection in Humans

Secretory Immune Response to Membrane Antigens during Giardia lamblia Infection in Humans,DISNEY M. ROSALES-BORJAS,JUAN DIAZ-RIVADENEYRA,ANTONIO DONA-

Secretory Immune Response to Membrane Antigens during Giardia lamblia Infection in Humans   (Citations: 3)
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The secretory immune response in humans infected with Giardia lamblia was studied by using saliva samples and a membrane-rich protein fraction. The membrane fraction, studied by sodium dodecyl sulfate-polyacryl- amide gel electrophoresis, showed 24 antigen bands, ranging from 170 to 14 kDa. Saliva samples from giardiasis patients showed a heterogeneous response against the membrane fraction when they were assayed by immunoblotting. Among the antigens recognized by patient saliva samples, those of 170, 105, 92, 66, 32, 29, and 14 kDa stood out. These antigens were not recognized by saliva samples from healthy individuals. They may be of importance in future studies of protection from or diagnosis of G. lamblia infections. Giardia lamblia is an important human pathogen of world- wide distribution (17, 22). G. lamblia transmission can be from person to person (12) but is more commonly waterborne, a result of the relative resistance of G. lamblia cysts to chlorina- tion (9). Manifestations of the disease vary from asymptomatic carriage to severe diarrhea and malabsorption. Host factors are thought to be important in determining the severity of the response to this parasite. Immune responses to this protozoan pathogen play a major role in determining the natural history of this infection and in the eventual development of protective immunity (3, 15). Since trophozoites do not appear to invade tissues, mucosal surfaces remain stimulated by Giardia anti- gens during the entire life span of the parasite. In this case, immunity to G. lamblia is closely associated with the type of immune response generated by mucosa-associated lymphoid tissue (6). Knowledge of the antigenic composition of the par- asite and the role that these antigens play in the immune response during infection is important for understanding the pathogenesis of the disease. Likewise, the identification of antigens recognized by the host immune system is of interest for understanding the modulation of G. lamblia infection. In this regard, surface membrane or plasma membrane antigens of G. lamblia seem to be more important because they very likely interact first with the host immune system. In this study, we examined the secretory immune response (SIR) during natural infection to G. lamblia membrane fractions by using saliva samples from patients with giardiasis and immunoblot techniques.
Published in 1998.
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    • ...In a previous paper, we described the specific immunoglobulin A (IgA) secretory response to membrane-associated antigens in a G. lamblia infection (28)...
    • ...The search for IgA antibodies to G. lamblia with an ELISA was performed essentially as previously described (28) by using 1 go f af finity-purified G. lamblia antigen per 50 l of carbonate buffer (pH 8.3) and 50-l portions of serial dilutions of saliva samples, followed by addition of peroxidase-conjugated affinity-purified goat anti-human IgA at a 1:1,600 dilution and finally o-phenylenediamine dihydrochloride (Sigma) and 10 l of 30% H2O2 ...
    • ...Previously (28), some of us determined the molecular masses of antigens recognized by the sIgA in an enriched Giardia membrane antigenic fraction...
    • ...The 8-kDa antigens appear to be the same as the 14-kDa antigen described by Rosales-Borjas et al. (28) and to have a frequency of 70% in positive samples and 100% specificity...

    S. M. T. Hasanet al. Human Secretory Immune Response to Fatty Acid-Binding Protein Fraction...

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