Academic
Publications
Desarrollo y validación de métodos analíticos para la cuantificación de antirretrovirales por HPLC

Desarrollo y validación de métodos analíticos para la cuantificación de antirretrovirales por HPLC,10.1016/S1130-6343(06)74009-0,Farmacia Hospitalaria

Desarrollo y validación de métodos analíticos para la cuantificación de antirretrovirales por HPLC  
BibTex | RIS | RefWorks Download
Summary Objective: Development, validation and error characteriza- tion of three analytical methods, by high performance liquid chro- matography (HPLC), for the quantitative analysis of ritonavir, saquinavir and abacavir in human plasma. Method: Reagents and instrumentation used, preparation of different standards, sample extraction procedure from biologic matrix, and analytical conditions assayed were detailed to set up three analytical methods. In addition, the validation and the deter- mination of analytical error were also described. Results: The analytical methods developed for ritonavir, saquinavir and abacavir in human plasma were selective, linear (r 2 > 0.99), precise (coefficients of variation < 15%) and accurate (relative errors < 15%) over the concentration range selected. The recovery was more than 95% in all methods. Antiretroviral drugs were stable in the storage conditions assayed according to the routine laboratory. The error function discriminated for each ana- lytical method validated was linear in saquinavir (SD = 4.84 + 7.14·10-
Journal: Farmacia Hospitalaria , vol. 30, no. 6, pp. 374-378, 2006
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.