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A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA

A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA,A. Gargas,P. T. Depriest

A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA   (Citations: 62)
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Published in 1996.
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    • ...The primer set NS23 and NS24 was used to amplify a 600–800-bp fragment of the fungal small-subunit rDNA [11]...

    Christian Pollet al. Small-scale Diversity and Succession of Fungi in the Detritusphere of ...

    • ...Beads were subsequently used to amplify fungal nuclear rDNA sequences (using nu-SSU-1203-5′ primers; Gargas and DePriest 1996) and internal transcribed spaser sequences (ITS4, using primers described by White et al. 1990 )i n polymerase chain reactions (PCR) using the following program: 94°C for 300 s, followed by five cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 60 s, 30 cycles of 94°C for 30 s, 52°C for ...
    • ... by White et al. 1990 )i n polymerase chain reactions (PCR) using the following program: 94°C for 300 s, followed by five cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 60 s, 30 cycles of 94°C for 30 s, 52°C for 30 s and 72°C for 60 s, with a final extension step of 72°C for 300 s. The PCR products were purified using a Viogene PCR-M Clean-up System and sequenced using PCR primers and the primers ...

    Benedicte R. Albrectsenet al. Endophytic fungi in European aspen ( Populus tremula ) leaves—diversit...

    • ...Primers used for the sequencing of the 18S rDNA and 28S rDNA were NS1 and NS8 (44); nu-SSU-402-5, nu-SSU-819-5, nu-SSU-852-3, and nu-SSU-305-3 (18); and LR1, LROR, LR21, LR2R, LR3, and LR5 (42)...

    Christiane Baschienet al. In Situ Detection of Freshwater Fungi in an Alpine Stream by New Taxon...

    • ...Scale is in base pairs according to the extension of the Gargas and DePriest [23] nomenclature system described in this study...
    • ...Nomenclature after Gargas and DePriest [23] as extended in this study...
    • ...The new suite of primers for Dikaryomycota presented here (specified using an extension of the Gargas and DePriest [23] numbering system) is anticipated to serve as the basis for a wide ranging system to analyze microbial communities, particularly in association with plants...
    • ...Nomenclature developed by Gargas and DePriest [23] for fungal primers was used with the exception that the sequence they refer to covers only the 1798 bases of 18S...

    Kendall J Martinet al. Fungal-specific PCR primers developed for analysis of the ITS region o...

    • ...The Group I introns identified in this study occurred at insertion sites previously reported in ascomycetes (Gargas et al.1995, Gargas & DePriest 1996, Perotto et al. 2000, Hambleton et al. 2005–this volume)...

    Sarah Hambletonet al. Meliniomyces, a new anamorph genus for root-associated fungi with phyl...

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