Cellulose orientation determines mechanical anisotropy in onion epidermis cell walls

Cellulose orientation determines mechanical anisotropy in onion epidermis cell walls,10.1093/jxb/erj177,Journal of Experimental Botany,D. Suslov

Cellulose orientation determines mechanical anisotropy in onion epidermis cell walls   (Citations: 14)
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The role of cellulose microfibril orientation in determin- ing cell wall mechanical anisotropy and in the control of the wall plastic versus elastic properties was studied in the adaxial epidermis of onion bulb scales using the constant-load (creep) test. The mean or net cellulose orientation in the outer periclinal wall of the epidermis was parallel to the long axis of the cells. In vitro cell wall extensibility was 30-90% higher in the direction per- pendicular to the net microfibril orientation than parallel to it. This was the case for the size of the initial de- formation occurring just after the load application and for the rate of time-dependent creep. Loading/unloading experiments confirmed the presence of a real irrevers- ible component in cell wall extension. The plastic com- ponent of the time-dependent deformation was higher perpendicular to the net cellulose orientation than par- allel to it. An acid buffer (pH 4.5) increased the creep rate by 25-30% but this response was not related to cellulose orientation. The present data provide direct evidence that the net orientation of cellulose micro- fibrils confers mechanical anisotropy to the walls of seed plants, a characteristic that may be relevant to understanding anisotropic cell growth.
Journal: Journal of Experimental Botany - J EXP BOT , vol. 57, no. 10, pp. 2183-2192, 2006
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    • ...Cell wall anisotropy and the mechanics of cellular growth In cells growing diffusely, the orientation of cellulose microfibrils is known to determine the direction of cell expansion (Baskin et al., 1999; Sugimoto et al., 2000) since it defines mechanical anisotropy in the cell walls (Burk and Ye, 2002; Suslov and Verbelen, 2006)...

    Leila Aouaret al. Morphogenesis of complex plant cell shapes: the mechanical role of cry...

    • ...However, recently, Suslov and Verbelen (2006) found elastic and plastic deformation when performing in vitro creep assays on onion epidermal cell walls...
    • ...Suslov and Verbelen (2006) argue that a complete unloading would correspond to a cell without turgor pressure and this in fact implies the complete absence of stresses, which is physiologically irrelevant...
    • ...Consistently, the authors could show that acid conditions increased the creep rate but did not affect the initial deformation when the load was applied (Suslov and Verbelen 2006)...

    Ingo Burgertet al. Mechanics of the Expanding Cell Wall

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