Simple Microplate Method for Determination of Urinary Iodine

Simple Microplate Method for Determination of Urinary Iodine,Toshinori Ohashi,Mitsuo Yamaki,Chandrakant S. Pandav,Madhu G. Karmarkar,Minoru Irie

Simple Microplate Method for Determination of Urinary Iodine   (Citations: 40)
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Background: Urinary iodine is a good biochemical marker for control of iodine deficiency disorders. Our aim was to develop and validate a simple, rapid, and quantitative method based on the Sandell-Kolthoff re- action, incorporating both the reaction and the digestion process into a microplate format. Methods: Using a specially designed sealing cassette to prevent loss of vapor and cross-contamination among wells, ammonium persulfate digestion was performed in a microplate in an oven at 110 °C for 60 min. After the digestion mixture was transferred to a transparent mi- croplate and the Sandell-Kolthoff reaction was per- formed at 25 °C for 30 min, urinary iodine was measured by a microplate reader at 405 nm. Results: The mean recovery of iodine added to urine was 98% (range, 89 -109%). The theoretical detection limit, defined as 2 SD from the zero calibrator, was 0.11 mmol/L (14 mg/L iodine). The mean intra- and interassay CVs for samples with iodine concentrations of 0.30 -3.15 mmol/L were <10%. The new method agreed well with the conventional chloric acid digestion method (n 5 70; r 5 0.991; y 5 0.944x 1 0.04; Syzx 5 0.10) and with the inductively coupled plasma mass spectrometry method (n 5 61; r 5 0.979; y 5 0.962x 1 0.03; Syzx 5 0.20). The agreement was confirmed by difference plots. The dis- tributions of iodine concentrations for samples from
Published in 2000.
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