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Microsatellite Variation Among Red Snapper ( Lutjanus campechanus ) from the Gulf of Mexico

Microsatellite Variation Among Red Snapper ( Lutjanus campechanus ) from the Gulf of Mexico,10.1007/s10126-001-0004-7,Marine Biotechnology,John R. Go

Microsatellite Variation Among Red Snapper ( Lutjanus campechanus ) from the Gulf of Mexico   (Citations: 17)
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:   Allelic variation at a total of 20 nuclear-encoded microsatellites was examined among adult red snapper (Lutjanus campechanus) sampled from 4 offshore localities in the Gulf of Mexico. The number of alleles at the 20 microsatellites ranged from 5 to 20; average (� SE) direct count heterozygosity values ranged from 0.148 � 0.025 to 0.902 � 0.008. No significant departures from expectations of Hardy-Weinberg equilibrium were found for any locus within samples, and genotypes at pairs of microsatellites appeared to be randomly associated, i.e., in genotypic equilibrium. Tests of homogeneity in allele distributions among the 4 localities were nonsignificant for 19 of the microsatellites. Allele distribution at microsatellite Lca 43 was heterogeneous among localities before (but not after) Bonferroni corrections for multiple tests executed simultaneously. Tests of homogeneity in the distribution of individual alleles at Lca 43 gave similar results: one low frequency allele was distributed heterogeneously among samples before, but not after, Bonferroni correction. Molecular analysis of variance indicated that more than 99% of variation at each microsatellite was distributed within sample localities. These results generally are consistent with the hypothesis of a single population (stock) of red snapper in the northern Gulf of Mexico.
Journal: Marine Biotechnology - MAR BIOTECHNOLOG , vol. 3, no. 3, pp. 293-304, 2001
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    • ...Primers used to amplify individual microsatellites were among those developed by Gold et al. (2001) for red snapper L...

    John R. Goldet al. Genetic Divergence and Effective Size among Lane Snapper in U.S. Water...

    • ...Polymerase-chain-reaction (PCR) primers used to amplify individual microsatellites were among those developed by Gold et al. (2001) for Gulf red snapper (L. campechanus) and by Bagley and Geller (1998) for vermilion snapper (Rhomboplites aurorubens)...

    S. Karlssonet al. Population structure and genetic variation of lane snapper ( Lutjanus ...

    • ...Gold et al. (2001) reported similar findings based on nuclear DNA microsatellite distributions...
    • ...Collectively, results reported by Fischer et al. (2004) and Woods et al. (2003) indicate significant variability in GOM red snapper population dynamics and demographics exists among regions, despite lack of genetic divergence in selectivelyneutral markers (Gold et al. 2001; Pruett et al. 2005; Saillant and Gold 2006)...

    William F. Patterson IIIet al. Temporal and Spatial Variability in Juvenile Red Snapper Otolith Eleme...

    • ...PCR primer sets for microsatellitesin all three species were developed previously for use in stock-structure analysis of ‘wild’ populations: red drum (Saillant et al., 2004); red snapper (Bagley and Geller, 1998; Gold et al., 2001), and cobia (Pruett et al., 2005; Renshaw et al., in press)...
    • ...Because these data already were available for red drum, red snapper, and cobia (Gold et al., 2001; Saillant et al., 2004; Renshaw et al., in press), the initial effort was conceptual and involved combining individual microsatellites with similar, optimal annealing temperatures and assessing whether allele-size ranges overlapped and whether alternate ABI dyes could be employed when overlap occurred...
    • ...Other PCR reagents (buffer, magnesium, dNTPs, Taq polymerase) in the ‘mega-cocktails’ initially followed PCR procedures outlined in Saillant et al. (2004), Gold et al. (2001), and Renshaw et al. (in press) for red drum, red snapper, and cobia, respectively...
    • ...PCR primer sequences for microsatellites with prefixes Lca and Prs may be found in Gold et al. (2001)...

    Mark A. Renshawet al. Microsatellite multiplex panels for genetic studies of three species o...

    • ...Lacking of population substructuring in L. campechanus was latter confirmed by microsatellite analysis (Gold, Pak & Richardson, 2001)...

    Pedro Manoel Galetti Junioret al. Assessing Genetic Diversity of Brazilian Reef Fishes by Chromosomal an...

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