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Evaluation of Eight Cow-Side Ketone Tests in Milk for Detection of Subclinical Ketosis in Dairy Cows

Evaluation of Eight Cow-Side Ketone Tests in Milk for Detection of Subclinical Ketosis in Dairy Cows,10.3168/jds.S0022-0302(00)74877-6,Journal of Dair

Evaluation of Eight Cow-Side Ketone Tests in Milk for Detection of Subclinical Ketosis in Dairy Cows   (Citations: 38)
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The objective of this study was to evaluate eight cow- side ketone tests when used with milk for detection of subclinical ketosis. A total of 469 dairy cows in the first week of lactation were studied. Twelve percent of these cows had subclinical ketosis, defined as >1400 µmol of β-hydroxybutyrate/L of blood serum. The Pink test liquid and the Ketolac test strip were highly sensitive for subclinical ketosis when used with milk. The Uris- can and Rapignost test strips were poorly sensitive; the Ketostix, Ketur-Test, and Medi-Test-Keton test strips and the Acetonreagenz test tablet were insensitive for subclinical ketosis when used with milk. Pink and Ke- tolac milk ketone tests are potentially useful tools for use in a routine monitoring program to detect subclini- cal ketosis in early postpartal dairy cows.
Journal: Journal of Dairy Science - J DAIRY SCI , vol. 83, no. 2, pp. 296-299, 2000
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    • ...Univariable data analysis and conventional logistic regression was conducted using Minitab v15. Further model exploration and posterior predictions were carried out using WinBUGS v1.4.1. (Imperial College and Medical Research Council). Each week, cows were split into groups with either a high or a normal metabolite recording. NEFA values were considered to be elevated if they were 0.4 mmol/l or more, BHB values were considered to be elevated if they were 1.4 mmol/l or more, indicating subclinical ketosis, and urea values were considered to be elevated if they were 7.5 mmol/l or more. Thresholds for NEFA, BHB and urea were chosen to correspond with those commonly used in practice and that were frequently quoted in the literature (...

    R. A. Jacksonet al. Energy metabolites in pre- and postpartum dairy cattle as predictors o...

    • ...A concentration of plasma BHBA greater than 1200 μmol/L is a common standard used for diagnosis of ketosis (Duffield 2000; Geishauser et al. 2000)...

    Chuang Xuet al. Comparative proteomic analysis of livers from ketotic cows

    • ...Milk BHBA concentration was determined usingapreviouslyvalidatedmilktest(KetoTest;Elanco Animal Health; Guelph, ON; Geishauser et al., 2000) between the second and eighth day postcalving and again 7 d later...
    • ...Twenty-six percent of animals (n = 1,041; 95% CI: 23.6 to 29.1) were subclinically ketotic (SCK; milk BHBA ≥100 mol/L; Geishauser et al., 2000) in the first week postpartum...

    R. B. Walshet al. Prevalence and Risk Factors for Postpartum Anovulatory Condition in Da...

    • ...The peak prevalence of SCK occurs in the second or third week after calving (Geishauser et al., 2000)...

    M. Sakhaet al. Bovine Subclinical Ketosis in Dairy Herds in Iran

    • ...A semiquantitative milk ketone test (KetoTest, Elanco Animal Health; Guelph Ontario, Canada) validated for BHBA (Geishauser et al., 2000) was used once during the first week and again during the second week postpartum...

    R. B. Walshet al. Synchronization of Estrus and Pregnancy Risk in Anestrous Dairy Cows A...

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