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Type X collagen synthesis during in vitro development of chick embryo tibial chondrocytes

Type X collagen synthesis during in vitro development of chick embryo tibial chondrocytes,10.1083/jcb.102.6.2310,Journal of Cell Biology,Patrizio Cast

Type X collagen synthesis during in vitro development of chick embryo tibial chondrocytes   (Citations: 50)
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In the developing chick embryo tibia type X collagen is synthesized by chondrocytes from re- gions of hypertrophy and not by chondrocytes from other regions (Capasso, O., G. Tajana, and R. Can- cedda, 1984, Mol. Cell. Biol. 4:1163-1168; Schmid, T. M., and T. F. Linsenmayer, 1985, Dev. Biol. 107:375- 381). To investigate further the relationship between differentiation of endochondral chondrocytes and type X collagen synthesis we have developed a novel cul- ture system for chondrocytes from 29-31-stage chick embryo tibiae. At the beginning of the culture these chondrocytes are small and synthesize type II and not type X collagen, but when grown on agarose-coated dishes they further differentiate into hypertrophic chondrocytes that synthesize type X collagen. The synthesis of type X collagen has been monitored in cultured cells by analysis of labeled collagens and in vitro translation of mRNAs. When the freshly disso- ciated chondrocytes are plated in anchorage-permis- sive dishes, most of the cells attach and dedifferen- tiate, as revealed by their fibroblastic morphology. De- differentiated chondrocytes, after several passages, can still reexpress the differentiated phenotype and con- tinue their development to hypertrophic, type X colla- gen-synthesizing chondrocytes. Hypertrophic chon- drocytes, when plated in anchorage permissive dishes, attach, maintaining the differentiated phenotype, and continue the synthesis of type X collagen.
Journal: Journal of Cell Biology - J CELL BIOL , vol. 102, no. 6, pp. 2310-2317, 1986
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