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Mass culture of hydra: an improved method and its application to other aquatic invertebrates

Mass culture of hydra: an improved method and its application to other aquatic invertebrates,10.1258/002367770781036463,Laboratory Animals,Howard M. L

Mass culture of hydra: an improved method and its application to other aquatic invertebrates   (Citations: 52)
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SUMMARY Virtually unlimited numbers of hydra can be grown under precise con- ditions in the laboratory with relative ease. Artemia cysts must be treated to remove gross contamination before hatching the nauplii to be used as the food of hydra. Large volumes of a variety of chemically defined culture solutions should be prepared. A particular regimen of animal care es- pecially devised for aquatic invertebrates must be adhered to. General requirements for, and the advantages of, laboratory cultivation of aquatic invertebrates are discussed. After methods for growing hydra in the laboratory had been devised (Loomis, 1953, 1954; Loomis & Lenhoff, 1956), this invertebrate was grown success- fully in numerous laboratories throughout the world and became the object of research on such subjects as behaviour, cell ultrastructure, development, and symbiosis. Since then experience has taught us how subtle changes in the aqueous environment can affect many aspects of the biology of hydra (Lenhoff, 1965b, 1968), and has led to improvements in these mass culture methods. Key steps for successful cultivation are 3. (1) Artemia nauplii derived from disinfected cysts are used as the food for the hydra, thus avoiding massive contamination of the cultures by bacteria and fungi. (2) Large volumes of culture solution are prepared from distilled-deionized water using proper amounts of added salts. (3) A regimen of daily care is followed which is rapid, simple, and safeguarded from error. Thus it is possible for an in- experienced person to culture hundreds of thousands of animals daily with relatively little effort.
Journal: Laboratory Animals - LAB ANIMALS , vol. 4, no. 1, pp. 139-154, 1970
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