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ORGANOGÉNESIS INDIRECTA Y ENRAIZAMIENTO in vitro DE Paulownia elongata

ORGANOGÉNESIS INDIRECTA Y ENRAIZAMIENTO in vitro DE Paulownia elongata,Osvaldo A. Castellanos-Hernández,Araceli Rodríguez-Sahagún,José M. Rodríguez-Do

ORGANOGÉNESIS INDIRECTA Y ENRAIZAMIENTO in vitro DE Paulownia elongata  
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In this study, the effect of three factors on indirect organogenesis of Paulownia elongata was evaluated. Explants were exposed for four weeks to different treatments, which were obtained by combining: Factor I, culture media: MS and WPM. Factor II, growth regulators: 5 and 10 mg·1 -1 BA, 0.5 mg·l -1 NAA ,1a nd 8m g·l -1 TDZ, 0.1 mg·1 -1 KIN. Fator III, type of explant: basal portion of leaf including petiole (P) and the central portion of leaf blades (C). The effect of different rooting agents was also evaluated for adventitious root formation on the regenerated shoots, by testing a chemical agent (1.5 mg·l-1 IBA), a physical agent (red light at 660 nm), and a biological agent (Agrobacterium rhizogenes). The best treatment for the production of shoots was the MS medium supplemented with 10 mg·l -1 BA and 0.5 mg·l-1 NAA and explant (P) in which 80% of the explants produced shoots. Plant growth was evaluated by measuring number of roots, root length, total plant height, stem thickness, and number of leaves. For adventitious root formation the chemical agent (1.5 mg·l -1 IBA) was the most effective treatment. It also was the number of leaves, while the physical agent and the control had similar data on root length, also, the chemical agent and the control had similar effects on stem thickness.
Published in 2006.
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