Platelet Shape Changes and Adhesion Under High Shear Flow

Platelet Shape Changes and Adhesion Under High Shear Flow,Mitsuhiro Kuwahara,Mitsuhiko Sugimoto,Shizuko Tsuji,Hideto Matsui,Tomohiro Mizuno,Shigeki Mi

Platelet Shape Changes and Adhesion Under High Shear Flow   (Citations: 9)
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Recent studies have revealed that the platelet adhesive process under flow is tightly regulated by multiple ligand-receptor interactions. However, platelet morphological changes during this process, particularly its physiological relevance, remain unknown under blood flow conditions. Using epifluorescence and scanning electron microscopy, we evaluated the real-time changes in platelet morphology during a platelet adhesive process on a von Willebrand factor- coated surface under physiological high shear flow in a perfusion chamber. Here, we show that dynamic platelet shape changes occurring during distinct phases of the adhesive process are precisely regulated by "inside-out" and "outside-in" integrin signals and are also a key regulatory element in successful platelet thrombogenesis opposing rapid blood flow in vivo. (Arterioscler Thromb Vasc Biol. 2002;22:329-334.)
Published in 2010.
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    • ...Regarding the activation mechanisms during the adhesive process on immobilized vWF, cytosolic calcium changes or shape changes in individual platelets have also been studied under high shear [22,27,28]...
    • ...Although it remains to be determined whether or not the cytosolic calcium elevation in platelets occurs during rolling by the inside-out signal from the vWF-GP Ib� interaction, the drastic calcium influx from the extracellular sources is thought to be established after firm adhesion by the outside-in signal from vWF binding to integrin � IIb� 3 [27,28]...
    • ...After firm adhesion and drastic calcium influx, the platelet adhesive process is finalized by the extensive spreading of platelets on a thrombogenic surface, thus providing a more potent adhesion and a larger thrombogenic base that are essential for the subsequent thrombus development [28]...

    Mitsuhiko Sugimotoet al. Functional Property of von Willebrand Factor Under Flowing Blood

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