Academic
Publications
Distinct Phases of siRNA Synthesis in an Endogenous RNAi Pathway in C. elegans Soma

Distinct Phases of siRNA Synthesis in an Endogenous RNAi Pathway in C. elegans Soma,10.1016/j.molcel.2010.01.012,Molecular Cell,Jonathan I. Gent,Ayele

Distinct Phases of siRNA Synthesis in an Endogenous RNAi Pathway in C. elegans Soma   (Citations: 16)
BibTex | RIS | RefWorks Download
SUMMARY Endogenous RNA-directed RNA polymerases (RdRPs) are cellular components capable of synthe- sizing new complementary RNAs from existing RNA templates. We present evidence for successive engagement of two different RdRPs in an endoge- nous siRNA-based mechanism targeting specific mRNAs in C. elegans soma. In the initiation stage of this process, a group of mRNA species are chosen as targets for downregulation, leading to accumula- tion of rare 26 nt 50-phosphorylated antisense RNAs that depend on the RdRP homolog RRF-3, the Argo- naute ERGO-1, DICER, and a series of associated (''ERI'') factors. This primary process leads to production of a much more abundant class of 22 nt antisense RNAs, dependent on a secondary RdRP (RRF-1) and associating with at least one distinct Argonaute (NRDE-3). The requirement for two RdRP/ Argonaute combinations and initiation by a rare class of uniquely structured siRNAs in this pathway illus- trate the caution and flexibility used as biological systems exploit the physiological copying of RNA.
Journal: Molecular Cell - MOL CELL , vol. 37, no. 5, pp. 679-689, 2010
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by: