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Frequent Loss of Imprinting of PEG1/MEST in Invasive Breast Cancer

Frequent Loss of Imprinting of PEG1/MEST in Invasive Breast Cancer,Inge S. Pedersen,Peter A. Dervan,Dennise Broderick,Michele Harrison,Nicola Miller,E

Frequent Loss of Imprinting of PEG1/MEST in Invasive Breast Cancer   (Citations: 20)
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Inge S. Pedersen, Peter A. Dervan, Dennise Broderick, Michele Harrison, Nicola Miller, Emma Delany, Donal O'Shea, Paul Costello, Alo McGoldrick, George Keating, Brendan Tobin, Tom Goreyhttp://academic.research.microsoft.com/io.ashx?type=5&id=12081384&selfId1=0&selfId2=0&maxNumber=12&query=
The human PEG1 gene is a newly identified imprinted gene on 7q32. Genetic aberrations of this chromosomal region are often detected in invasive breast carcinomas. In this study, we show monoallelic PEG1 expression in normal breast tissue, indicating the presence of a functional imprint, and more importantly, we demonstrate loss of imprinting (LOI) in all of seven informative invasive breast carcinomas. In contrast to this, in one case of atypical ductal hyperplasia (ADH) found in residual breast, imprinting was maintained. This raises the possibility that aberrant im- printing of PEG1 may be involved in the progression from hyperplasia to invasive breast cancer.
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    • ...These studies investigated the LOI of the insulin-like growth factor 2 (IGF2) gene and the H19 gene, a well-known genomic imprinting gene. In terms of PEG1/MEST, LOI of PEG1/MEST was also detected in lung, breast and colon cancer [...

    Yong-Suk Moonet al. Imprinting and Expression Status of Isoforms 1 and 2 of PEG1/MEST Gene...

    • ..., and MEST, a known target of hypomethylation and loss of imprinting in breast cance...

    Christine Ladd-Acostaet al. The human colon cancer methylome shows similar hypo- and hypermethylat...

    • ..., and PEG1 (also known as MEST) in breast cance...

    Andrew P. Feinberg. Phenotypic plasticity and the epigenetics of human disease

    • ...fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=4232">MEST) gene in breast, lung, and colon cance...

    Keith D. Robertson. DNA methylation and human disease

    • ...PEG1/MEST in infiltrating carcinomas of the breast (9), an alternative non-imprinted PEG1/MEST transcript has been isolated from human adult blood lymphocytes (10)...
    • ...The monoallelic PEG1/MEST expression reported previously (9), when investigating the common 30-UTR directly, reflects the allelic usage of isoform 1. This indicates that isoform 1 is the predominant transcript in normal breast tissue...
    • ...Owing to the fact that isoform 2 is expressed either biallelically or from the opposite allele to isoform 1, the possibility exists that the biallelic profiles of the breast carcinomas detected originally (9) is a result of a switch in expression from isoform 1 to isoform 2. To investigate this further, we established the relative expression levels of the two isoforms by isoform-specific realtime RT‐PCR...
    • ...All samples analysed (both normal and cancer) had a monoallelic profile, ruling out LOI of isoform 1 as the cause of the biallelic PEG1/MEST expression detected originally (9)...
    • ...However, we know from our previous study that the infiltrating tumour lymphocytes are unlikely to influence the expression profiling (9)...
    • ...Six of the seven samples originated from the cohort described in our previous work (9)...
    • ...The purified RT‐PCR products were subsequently used as template in a nested PCR and digested with AflIII restriction enzyme (GibcoBRL) as previously described (9,16)...
    • ...The restriction profiles were evaluated by densitometry (9)...

    Inge Søkilde Pedersenet al. Promoter switch: a novel mechanism causing biallelic PEG1/MEST express...

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