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Abundance of SSR Motifs and Development of Candidate Polymorphic SSR Markers (BARCSOYSSR_1.0) in Soybean

Abundance of SSR Motifs and Development of Candidate Polymorphic SSR Markers (BARCSOYSSR_1.0) in Soybean,10.2135/cropsci2009.10.0607,Crop Science,Qiji

Abundance of SSR Motifs and Development of Candidate Polymorphic SSR Markers (BARCSOYSSR_1.0) in Soybean   (Citations: 7)
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Simplesequencerepeat� (SSR)� geneticmark- ers,�alsoreferredtoasmicrosatellites,�function� inmap-basedcloningandformarker-assisted� selectioninplantbreeding.� Theobjectivesof� thisstudyweretodeterminetheabundanceof� SSRsinthesoybeangenomeandtodevelop� andtestsoybeanSSRmarkerstocreatea� databaseoflocus-specificmarkerswithahigh� likelihoodofpolymorphism.� Atotalof� 210,990� SSRswithdi-,�tri-,�andtetranucleotiderepeats� offiveormorewereidentifiedinthesoybean� wholegenomesequence�(WGS)�whichincluded� 61,458� SSRsconsistingofrepeatunitsofdi-� (³10),�tri-�(³8),�andtetranucleotide�(³7).�Among� the� 61,458� SSRs,� (AT)n,� (ATT)nand� (AAAT)n� werethemostabundantmotifsamongdi-,�tri-,� andtetranucleotideSSRs,� respectively.� After� screeningforanumberoffactorsincluding� locus-specificityusinge-PCR,� asoybeanSSR� database�(BARCSOYSSR_1.0)�withthegenome�
Journal: Crop Science - CROP SCI , vol. 50, no. 5, 2010
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