Expression profile analysis of early fruit development in iaaM-parthenocarpic tomato plants

Expression profile analysis of early fruit development in iaaM-parthenocarpic tomato plants,10.1186/1756-0500-2-143,BMC Research Notes,Barbara Molesin

Expression profile analysis of early fruit development in iaaM-parthenocarpic tomato plants   (Citations: 4)
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BACKGROUND: Fruit normally develops from the ovary after pollination and fertilization. However, the ovary can also generate seedless fruit without fertilization by parthenocarpy. Parthenocarpic fruit development has been obtained in tomato (Solanum lycopersicum) by genetic modification using auxin-synthesising gene(s) (DefH9-iaaM; DefH9-RI-iaaM) expressed specifically in the placenta and ovules. FINDINGS: We have performed a cDNA Amplified Fragment Length Polymorphism (cDNA-AFLP) analysis on pre-anthesis tomato flower buds (0.5 cm long) collected from DefH9-iaaM and DefH9-RI-iaaM parthenocarpic and wild-type plants, with the aim to identify genes involved in very early phases of tomato fruit development. We detected 212 transcripts differentially expressed in auxin-ipersynthesising pre-anthesis flower buds, 65 of them (31%) have unknown function. Several differentially expressed genes show homology to genes involved in protein trafficking and protein degradation via proteasome. These processes are crucial for auxin cellular transport and signaling, respectively. CONCLUSION: The data presented might contribute to elucidate the molecular basis of the fruiting process and to develop new methods to confer parthenocarpy to species of agronomic interest. In a recently published work, we have demonstrated that one of the genes identified in this screening, corresponding to #109 cDNA clone, regulates auxin-dependent fruit initiation and its suppression causes parthenocarpic fruit development in tomato.
Journal: BMC Research Notes - BMC Res Notes , vol. 2, no. 1, pp. 143-7, 2009
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    • ...For instance, the chimeric gene DefH9-iaaM, which could promote the synthesis of IAA (Pandolfini et al. 2002; Molesini et al. 2009), has been used to confer parthenocarpic fruit development in several species belonging to different plant families (Mezzetti et al. 2004), and the agronomical advantages of DefH9-iaaM plants have been assessed in greenhouse and field trials (Yin et al. 2006; Giuseppe et al. 2005)...
    • ...In many plant species, fruit set can be improved by auxin manipulation, either chemically or genetically (Molesini et al. 2009; Uchiumi and Okamoto 2010)...

    Lei Wanget al. Functional analysis of an iaaM gene in parthenocarpic fruit developmen...

    • ...This technique was also applied to screen related genes expressed in different organs or in different developmental phase [36, 37]...

    Lei Xinget al. Characterization of Betula platyphylla gene transcripts associated wit...

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