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Spacer Oligonucleotide Typing ofMycobacterium bovisStrains from Cattle and Other Animals: a Tool for Studying Epidemiology of Tuberculosis

Spacer Oligonucleotide Typing ofMycobacterium bovisStrains from Cattle and Other Animals: a Tool for Studying Epidemiology of Tuberculosis,ALICIA ARAN

Spacer Oligonucleotide Typing ofMycobacterium bovisStrains from Cattle and Other Animals: a Tool for Studying Epidemiology of Tuberculosis   (Citations: 28)
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The spacer oligonucleotide typing (spoligotyping) method was evaluated for its ability to differentiate Mycobacterium bovisstrains. This method detects the presence or absence of spacers of the direct repeat locus of the M. bovis genome. The spacers in the direct repeat locus are amplified by PCR and are detected by hybridization of the biotin-labelled PCR product with a membrane containing oligonucleotides derived from spacer sequences that have previously been bound to a membrane. One hundred eighty-twoM. bovisisolates fromdomesticanimals(cattle,goat,sheep,andcats)andwildanimals(deerandwildboar)werespoligotyped, and the results were compared with those obtained by IS6110 restriction fragment length polymorphism analysis. Two rather homogeneous clusters of isolates containing 20 and 4 types, respectively, were identified by spoligotyping. The first cluster included isolates from cattle, cats, and feral animals. By spoligotyping, isolates from the Spanish wild boar and deer had the same pattern as some bovine isolates, suggesting transmissionbetweentheseanimalsandcattleandhighlightingtheimportanceofthestudyofthesereservoirs. Thesecondclusterincludedallthecaprineandovineisolates.Withineachcluster,thepatternsofthedifferent strains differed only slightly, suggesting that the spoligotypes may be characteristic of strains from particular animal species. Spoligotyping proved to be useful for studying the epidemiology of bovine M. bovis isolates, especially of those isolates containing only a single copy of IS6110. In view of our results, we suggest fingerprintingallM.bovisstrainsbythespoligotypingmethodinitiallyandthenbyIS6110restrictionfragment length polymorphism typing of the strains belonging to the most common spoligotypes.
Published in 1996.
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