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Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils

Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils,10.1128/AEM.69.6.3280-3287.2003,Applied and E

Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils   (Citations: 55)
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Biosurfactants are a unique class of compounds that have been shown to have a variety of potential applications in the remediation of organic- and metal-contaminated sites, in the enhanced transport of bacteria, in enhanced oil recovery, as cosmetic additives, and in biological control. However, little is known about the distribution of biosurfactant-producing bacteria in the environment. The goal of this study was to determine how common culturable surfactant-producing bacteria are in undisturbed and contaminated sites. A series of 20 contaminated (i.e., with metals and/or hydrocarbons) and undisturbed soils were collected and plated on R2A agar. The 1,305 colonies obtained were screened for biosurfactant production in mineral salts medium containing 2% glucose. Forty-five of the isolates were positive for biosurfactant production, repre- senting most of the soils tested. The 45 isolates were grouped by using repetitive extragenic palindromic (REP)-PCR analysis, which yielded 16 unique isolates. Phylogenetic relationships were determined by com- paring the 16S rRNA gene sequence of each unique isolate with known sequences, revealing one new biosur- factant-producing microbe, a Flavobacterium sp. Sequencing results indicated only 10 unique isolates (in comparison to the REP analysis, which indicated 16 unique isolates). Surface tension results demonstrated that isolates that were similar according to sequence analysis but unique according to REP analysis in fact produced different surfactant mixtures under identical growth conditions. These results suggest that the 16S rRNA gene database commonly used for determining phylogenetic relationships may miss diversity in micro- bial products (e.g., biosurfactants and antibiotics) that are made by closely related isolates. In summary, biosurfactant-producing microorganisms were found in most soils even by using a relatively limited screening assay. Distribution was dependent on soil conditions, with gram-positive biosurfactant-producing isolates tending to be from heavy metal-contaminated or uncontaminated soils and gram-negative isolates tending to be from hydrocarbon-contaminated or cocontaminated soils.
Journal: Applied and Environmental Microbiology - AEM , vol. 69, no. 6, pp. 3280-3287, 2003
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    • ...Isolates exhibited a relative reduction of surface tension for samples with biodiesel in aqueous solution when compared to the values of the surface tension of water (from 60 to 72 mN m−1)[ 25 ] and pure mineral medium (65 mN m−1)...

    Daniel Derrossi Meyeret al. Capability of a selected bacterial consortium for degrading diesel/bio...

    • ...In addition, glucose has been widely reported to support the production of a variety of biosurfactants, as a consequence of which there is growing interest in using the substrate (due to its nontoxicity) to stimulate biosurfactant production in situ for remediation applications (Desai and Banat, 1997; Bodour et al, 2003, 2004; Nitschke et al, 2004; Ilori et al, 2005; Amaral et al, 2006; Sarrubo et al, 2006)...

    O. A. Agwuet al. A Comparative Study of Biosurfactant Synthesis by Pseudomonas aerugino...

    • ...Biosurfactant-producing microorganisms have been isolated from different heavy metal- and oil- contaminated substrates, such as soil, lagoon water (Bodour et al. 2003; Ilori et al. 2005; Menezes-Bento et al. 2005), seawater and marine sediments (Olivera et al. 2009; Das et al. 2008; Yakimov et al. 1998)...

    Juan Carlos Velázquez-Aradillaset al. Characterisation of a biosurfactant produced by a Bacillus cereus stra...

    • ...In comparison with synthetic surfactants, biosurfactants possess distinct advantages which include biodegradability, generally low toxicity, ecological acceptability, ability to be produced from renewable resources, and functionality under extreme conditions (eg, high or low temperature and pH, high pressure, and salinity) (Bodour et al, 2003; Kosaric, 2001)...

    Hui Yuet al. Isolation and Characterization of Biosurfactant-and Bioemulsifier-Prod...

    • ...Most of the known lipopeptide-producing strains are Bacillus species (Bodour et al. 2003, Bonmatin et al. 2003, Liu et al. 2005, Raaijmakers et al. 2006, Strieker and Marahiel...
    • ...2003). Maier's group has reported that a REP (repetitive extragenic palindromic) analysis was adequate to distinguish unique biosurfactant-producing isolates, but that 16S rDNA PCR followed by sequencing was not (Bodour et al. 2003)...

    Xiangyang Liuet al. Production and characterization of a group of bioemulsifiers from the ...

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