Academic
Publications
Molecular Cloning of a Gene Encoding the Histamine H2 Receptor

Molecular Cloning of a Gene Encoding the Histamine H2 Receptor,10.1073/pnas.88.2.429,Proceedings of The National Academy of Sciences,Ira Gantz,Matthia

Molecular Cloning of a Gene Encoding the Histamine H2 Receptor   (Citations: 98)
BibTex | RIS | RefWorks Download
The H2 subclass of histamine receptors mediates gastric acid secretion, and antagonists for this receptor have proven to be effective therapy for acid peptic disorders of the gastrointestinal tract. The physiological action of histamine has been shown to be mediated via a guanine nucleotide-binding protein linked to adenylate cyclase activation and cellular cAMP generation. We capitalized on the technique of polymerase chain reaction, using degenerate oligonucleotide primers based on the known homology between cellular receptors linked to guanine nucleotide-binding proteins to obtain a partial-length clone from canine gastric parietal cell cDNA. This clone was used to obtain a full-length receptor gene from a canine genomic library. Histamine increased in a dose-dependent manner cellular cAMP content in L cells permanently transfected with this gene, and preincubation of the cells with the H2-selective antagonist cimetidine shifted the dose-response curve to the right. Cimetidine inhibited the binding of the radiolabeled H2 receptor-selective ligand [methyl-^3H]tiotidine to the transfected cells in a dose-dependent fashion, but the H1-selective antagonist diphenhydramine did not. These data indicate that we have cloned a gene that encodes the H2 subclass of histamine receptors.
Journal: Proceedings of The National Academy of Sciences - PNAS , vol. 88, no. 2, pp. 429-433, 1991
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
    • ...As is well known, histamine H2 receptors located on gastric cells promote the production of gastric acid [10] and histamine H2 receptor blockers have been widely used for the treatment of peptic ulcer...

    Hiroyuki Takahamaet al. A histamine H 2 receptor blocker ameliorates development of heart fail...

    • ...Specifically, because histamine H2 receptors are known to be located in gastric cells and enhance the production of acids that cause gastric ulcers, the blocker of histamine H2 receptors is developed as the drug for the treatment of gastric ulcers (7)...
    • ...Age (yrs) 66 16 6 1 M/F gender (%) 97/62 (61/39) 97/62 (61/39) Hypertension (%) 11 (7) 11 (7) Duration of CHF (yrs) 8.7 0.7 8.5 0.8 Systolic blood pressure (mm Hg) 112 9 105 8* Diastolic blood pressure (mm Hg) 67 46 2 5* Heart rate (beats/min) 73 56 6 5* Fractional shortening (%) 24 12 3 1 LV diastolic diameter (mm) 58 25 4 1* LV systolic diameter (mm) 44 14 1 1* LA diameter (mm) 40 33 9 3 Pressure across tricuspid valve (mm Hg) 30 22 8 2 ...
    • ...Age (yrs) 66 16 6 1 M/F gender (%) 97/62 (61/39) 97/62 (61/39) Hypertension (%) 11 (7) 11 (7) Duration of CHF (yrs) 8.7 0.7 8.5 0.8 Systolic blood pressure (mm Hg) 112 9 105 8* Diastolic blood pressure (mm Hg) 67 46 2 5* Heart rate (beats/min) 73 56 6 5* Fractional shortening (%) 24 12 3 1 LV diastolic diameter (mm) 58 25 4 1* LV systolic diameter (mm) 44 14 1 1* LA diameter (mm) 40 33 9 3 Pressure across tricuspid valve (mm Hg) 30 22 8 2 ...

    Jiyoong Kimet al. Impact of Blockade of Histamine H 2 Receptors on Chronic Heart Failure...

    • ... showed in 1999 that, like the H1 and H2 receptor...

    Remko A. Bakkeret al. The histamine H3 receptor: from gene cloning to H3 receptor drugs

    • ... Besides the well-known H1 and H2 histamine receptors, two additional types of histamine receptors have been described by molecular cloning of cDNAs encoding G protein coupled receptors ...

    Undine Lippertet al. Human Skin Mast Cells Express H2 and H4, but not H3 Receptors

Sort by: