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Analysis of Gene Expression Profiles in Leaf Tissues of Cultivated Peanuts and Development of EST-SSR Markers and Gene Discovery

Analysis of Gene Expression Profiles in Leaf Tissues of Cultivated Peanuts and Development of EST-SSR Markers and Gene Discovery,10.1155/2009/715605,I

Analysis of Gene Expression Profiles in Leaf Tissues of Cultivated Peanuts and Development of EST-SSR Markers and Gene Discovery   (Citations: 4)
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Journal: International Journal of Plant Genomics , vol. 2009, pp. 1-15, 2009
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    • ...The EST sequences were generated from various cDNA libraries (Guo et al. 2008; Guo et al. 2009) and collected from public database GenBank...
    • ...Several raw EST clones were picked out from cDNA libraries (Guo et al. 2008; Guo et al. 2009 )a nd screened by universal primers T3 and T7. Out of them, two clones...

    Xiaoping Chenet al. Identification and Characterization of a Multigene Family Encoding Ger...

    • ...In recent years, a large number of SSR markers for peanut have been developed from genomic DNA libraries and expressed sequence tags (EST) with the goal of providing sufficient sequence resources for developing a critical mass of DNA markers for the community [22,23], making it feasible to use SSRs to construct a genetic linkage map...
    • ...Therefore, the objective of this study was to construct a SSR-based composite reference map for cultivated peanut using public available SSR sources [13,19,24-28], GenBank [29] and newly developed ESTs [22,23]...

    Yanbin Honget al. A SSR-based composite genetic linkage map for the cultivated peanut (A...

    • ...In addition, microsatellite or simple sequence repeat (SSR) markers have been developed using sequences derived from SSR-enriched genomic libraries and expressed sequence tags (ESTs) (Hopkins et al. 1999; Palmieri et al. 2002, 2005; He et al. 2003, 2005; Ferguson et al. 2004; Moretzsohn et al. 2004, 2005; Mace et al. 2006; Gimenes et al. 2007; Proite et al. 2007; Cuc et al. 2008; Naito et al. 2008; Guo et al. 2009)...
    • ...The number of EST-SSR markers generated per non-redundant EST was 0.32, which was more than 7‐12 times higher than in previous reports (0.042, for 290 markers/6888 ESTs by Guo et al. 2009; and 0.025, for 290 markers/ 11431ESTs by Liang et al. 2009)...
    • ...This rate was higher than in some of the previous reports, which had polymorphism rates of 8.9% (Guo et al. 2009), 7.5% (Moretzsohn et al. 2005) and 10.3% (Liang et al. 2009), but less than that reported by Luo et al. (20%; 2005)...

    Padmalatha KoilkondaShuseiet al. Large-scale development of expressed sequence tag-derived simple seque...

    • ...The largest proportion of the unigene involved in the Metabolism pathway was expected since leaf tissues were used in the construction of the cDNA library, which is similar to that of ESTs from peanuts (Guo et al. 2009)...

    Z. M. Gaoet al. Generation and analysis of expressed sequence tags from a normalized c...

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