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Application of high Tc SQUID magnetometer to biological immunoassays

Application of high Tc SQUID magnetometer to biological immunoassays,10.1109/77.919431,IEEE Transactions on Applied Superconductivity,K. Enpuku,T. Min

Application of high Tc SQUID magnetometer to biological immunoassays   (Citations: 10)
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A high Tc SQUID system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. Design and set up of the system is described. Minimum detectable amplitude of the magnetic flux is 0.6 mΦ0 for the measurement bandwidth from 0.2 Hz to 5 Hz when we use a magnetometer. The system noise does not increase when the magnetic field of 0.8 mT is applied in parallel to the SQUID. An experiment to measure the antigen-antibody reaction shows that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. When a gradiometer is used, the system noise decreased by a factor of 5, compared to the case of the magnetometer. This improvement indicates the usefulness of the gradiometer to suppress the residual environmental noise in the present system. Magnetic markers that have remanent magnetic moment are also studied in order to increase the signal
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    • ...HTS-SQUID systems such as magnetocardiography [1], nondestructive evaluation [2], immunoassay [3], integrated circuit testing [4] etc...

    Seiji Adachiet al. Fabrication of Low-Noise HTS-SQUID Gradiometers and Magnetometers With...

    • ...Based on these magnetic properties, there are three primary methods for detection of the magnetic signal emanating from the MNPs: remanence (Enpuku et al 2003a, Kawagishi et al 2004, Kotitz et al 1997, Tsukamoto et al 2005), relaxation (Chemla et al 2000, Grossman et al 2004, Haller et al 2001, Lange et al 2002 ,L eeet al 2002) and susceptibility (Enpuku et al 1999, 2001, Tanaka et al 2003) measurements...

    Song Geet al. Development of a remanence measurement-based SQUID system with in-dept...

    • ...highly sensitive magnetometer system that utilizes a superconducting quantum interference device (SQUID) has been developed for the application to biological immunoassays [1]‐[5]...
    • ...The other is to measure the magnetization of the marker in the presence of the dc excitation field [4], [5]...
    • ...Typical measurement bandwidth is from 0.5 Hz to 10 Hz. Details of the system are described elsewhere [5]...
    • ...Note that the cancellation is easy in the case of the magnetometer since we can monitor with the magnetometer [5]...

    K. Enpukuet al. High Tc SQUID system and magnetic marker for biological immunoassays

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