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Hydrogen exchange mass spectrometry: what is it and what can it tell us?

Hydrogen exchange mass spectrometry: what is it and what can it tell us?,10.1007/s00216-010-3556-4,Analytical and Bioanalytical Chemistry,Sean R. Marc

Hydrogen exchange mass spectrometry: what is it and what can it tell us?   (Citations: 6)
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Proteins are undoubtedly some of the most essential molecules of life. While much is known about many proteins, some aspects still remain mysterious. One particularly important aspect of understanding proteins is determining how structure helps dictate function. Continued development and implementation of biophysical techniques that provide information about protein conformation and dynamics is essential. In this review, we discuss hydrogen exchange mass spectrometry and how this method can be used to learn about protein conformation and dynamics. The basic concepts of the method are described, the workflow illustrated, and a few examples of its application are provided. Figure Analysis of deuterium incorporation into protein with mass spectrometry
Journal: Analytical and Bioanalytical Chemistry - ANAL BIOANAL CHEM , vol. 397, no. 3, pp. 967-972, 2010
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