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Separation of desulphoglucosinolates by micellar electrokinetic capillary chromatography based on a bile salt

Separation of desulphoglucosinolates by micellar electrokinetic capillary chromatography based on a bile salt,10.1016/0021-9673(95)00535-8,Journal of

Separation of desulphoglucosinolates by micellar electrokinetic capillary chromatography based on a bile salt   (Citations: 9)
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Micellar electrokinetic capillary chromatography (MECC) has been developed as a promising method for the determination of 40 desulphoglucosinolates. A sodium cholate based MECC method was found to be efficient for the qualitative and quantitative analysis of desulphoglucosinolates produced in an on-colum, enzymatic step form the corresponding intact glucosinolates. Separation conditions and sensitivity of the method have been optimised with respect to different parameters, including capillary types, where the 75-μm I.D. capillary increased the sensitivity 2.5 times over that of a 50-μ capillary. With use of a high-sensitivity optical cell assembly (Z-cell), the sensitivity was further increased ten times, resulting in detection of picogram amounts, or concentration levels corresponding to 10−6M. Repeatability with a 75-μm capillary was good, with the relative standard deviation varying between 0.2% and 0.9% for relative migration times and for relative normalised areas between 1.0% and 3.0%. Linearity of the optimised method gave correlation coefficients between 0.99 and 0.9999 for the 50-μm capillary and 0.99 and 0.9997 for the 75-μm capillary. Separation efficiency expressed as number of theoretical plates (N/m) was in the range 250 000–300 000 for the 50-μm capillary and 210 000–250 000 for the Z-cell. Limitations and possibilities of the MECC method here presented are discussed with respect to analyses of glucosinolates occuring in a wide range of cruciferous seed, vegetative plant parts including cabbage varieties, feed and food.
Journal: Journal of Chromatography A - J CHROMATOGR A , vol. 717, no. 1, pp. 325-333, 1995
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