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Comprehensive blood plasma lipidomics by liquid chromatography/quadrupole time-of-flight mass spectrometry

Comprehensive blood plasma lipidomics by liquid chromatography/quadrupole time-of-flight mass spectrometry,10.1016/j.chroma.2010.02.039,Journal of Chr

Comprehensive blood plasma lipidomics by liquid chromatography/quadrupole time-of-flight mass spectrometry   (Citations: 6)
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A lipidomics strategy, combining high resolution reversed-phase liquid chromatography (RPLC) with high resolution quadrupole time-of-flight mass spectrometry (QqTOF), is described. The method has carefully been assessed in both a qualitative and a quantitative fashion utilizing human blood plasma. The inherent low technical variability associated with the lipidomics method allows to measure 65% of the features with an intensity RSD value below 10%. Blood plasma lipid spike-in experiments demonstrate that relative concentration differences smaller than 25% can readily be revealed by means of a t-test. Utilizing an advanced identification strategy, it is shown that the detected features mainly originate from (lyso-)phospholipids, sphingolipids, mono-, di- and triacylglycerols and cholesterol esters. The high resolution offered by the up-front RPLC step further allows to discriminate various isomeric species associated with the different lipid classes. The added value of utilizing a Jetstream electrospray ionization (ESI) source over a regular ESI source in lipidomics is for the first time demonstrated. In addition, the application of ultra high performance LC (UHPLC) up to 1200bar to extend the peak capacity or increase productivity is discussed.
Journal: Journal of Chromatography A - J CHROMATOGR A , vol. 1217, no. 25, pp. 4087-4099, 2010
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    • ...and high resolution reversed-phase LC (RPLC) with high resolution quadrupole time-of-flight mass spectrometry (QqTOF) ...

    Nikolaos Psychogioset al. The Human Serum Metabolome

    • ...Spectra were recorded on an LTQ-Orbitrap XL. Mass measurements were made to four decimal places although peaks are only annotated to two decimal places J Inherit Metab Dis (2011) 34:583–592 587 investigations (Sandra et al. 2010)...
    • ...In a recent study Sandra et al. were able to identify over 1500 features (retention time, m/z pairs) from a plasma lipid extract illustrating the potential of the methodology (Sandra et al. 2010)...

    William J. Griffithset al. On the future of “omics”: lipidomics

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