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Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection

Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection,10.1016/j.carbpol.2010.04.051,Carbohydrate Polymers,Shunsheng Ca

Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection   (Citations: 3)
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Shunsheng Cao, Rajeev Mishra, Srikanth Pilla, Swapnil Tripathi, Manoj K. Pandey, Gopit Shah, Ajay K. Mishra, Mani Prabaharan, Shivani B. Mishra, Jin Xin, R. R. Pandey, Weiwei Wuhttp://academic.research.microsoft.com/io.ashx?type=5&id=23341176&selfId1=0&selfId2=0&maxNumber=12&query=
A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid, Au-MPA NPs). The prepared CHIT/Au-MPA/ITO and the ssODNs/CHIT/Au-MPA/ITO electrodes were characterized with FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). TEM micrograph of the composite electrode showed that Au-MPA NPs had the diameter ranging from 4 to 16nm. The ssODNs/CHIT/Au-MPA/ITO electrode showed a linear increase in the amperometric current with an increasing concentration of the single-stranded target complementary ODNs (cODNs) within the range of 0.03–325fM. The ssODNs/CHIT/Au-MPA/ITO electrode exhibited a sensitivity of 0.572μA/fM with a response time of 12s. The ssODNs/CHIT/Au-MPA/ITO electrode had a shelf life of ∼182 days, when stored at 4°C.
Journal: Carbohydrate Polymers - CARBOHYD POLYM , vol. 82, no. 1, pp. 189-194, 2010
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