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Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly- N-acetyllactosamine antigens

Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly- N-acetyllactosamine antigens,10.1016/j.carres.2006.03.043,Carbohydrate

Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly- N-acetyllactosamine antigens   (Citations: 4)
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Poly-N-acetyllactosamines (pLNs) are common terminal sugars of many N- and O-linked glycan structures present in glycoproteins and glycolipids. Utilizing various glycosyltransferases, we developed new and efficient chemoenzymatic methods for the synthesis of pLNs in gram-scale. Specifically, the use of sialyltransferases and fucosyltransferases enabled us to synthesize and purify 24 blood group and tumor-associated pLN derivatives with α-(2→3)- and α-(2→6)-linked sialic acid, as well as with α-(1→2)- and α-(1→3)-linked fucose. All synthesized derivatives were linked to a short 2-azidoethyl spacer for further modification.
Journal: Carbohydrate Research - CARBOHYD RES , vol. 341, no. 10, pp. 1447-1457, 2006
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    • ...This was impressively demonstrated by Blixt et al. with a large-scale enzymatic synthesis of poly-LacNAc based glycan structures using bacterial enzyme systems including LgtA (β1-4galactosyltransferase, β4GalT) and LgtB from Neisseria meningitidis (β1-3N-acetyl-glucosaminyltransferase, β3GlcNAcT) [17, 28, 29]...
    • ...The use of the azidogroup for the immobilization to surfaces is well described [17]...

    Birgit Sauerzapfeet al. Chemo-enzymatic synthesis of poly- N -acetyllactosamine (poly-LacNAc)...

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