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Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells

Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells,10.1038/cddis.2010.14,Cell

Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells   (Citations: 2)
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The maintenance of viable and functional islets is critical in successful pancreatic islet transplantation from cadaveric sources. During the isolation procedure, islets are exposed to a number of insults including ischemia, oxidative stress and cytokine injury that cause a reduction in the recovered viable islet mass. A novel approach was designed in which streptozotocin (STZ)-damaged rat pancreatic islets (rPIs) were indirectly cocultured with rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to maintain survival of the cultured rPIs. The results indicated that islets cocultured with rBM-MSCs secreted an increased level of insulin after 14 days, whereas non-cocultured islets gradually deteriorated and cell death occurred. The cocultivation of rBM-MSCs with islets and STZ-damaged islets showed the expression of IL6 and transforming growth factor-β1 in the culture medium, besides the expression of the antiapoptotic genes (Mapkapk2, Tnip1 and Bcl3), implying the cytoprotective, anti-inflammatory and antiapoptotic effects of rBM-SCs through paracrine actions.
Journal: Cell Death and Disease , vol. 1, no. 4, 2010
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