Linking fluorescence measurements to radiometric units
A general concept is presented to realize the traceability of fluorescence measurements to radiometric units based on a combination of suited physical and chemical transfer standards and a reference fluorometer as a linking unit. The standards comprise a newly designed spectral radiance transfer standard more closely adapted to the spectral radiance of typical fluorescent samples than conventional standard lamps and a set of easy-to-operate spectral fluorescence standards consisting of organic dyes. An example is given as to how the comparability of fluorescence spectra across instruments can be improved. Finally, the design and the calibration scheme of a new reference fluorometer is presented which is designed for the performance of relative and absolute fluorescence measurements with minimum uncertainty.
, vol. 43, no. 2, pp. 89-S93, 2006