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Inhibition of the NF-κB transcription factor increases Bax expression in cancer cell lines

Inhibition of the NF-κB transcription factor increases Bax expression in cancer cell lines,10.1038/sj.onc.1204343,Oncogene,Mohamed Bentires-Alj,Emmanu

Inhibition of the NF-κB transcription factor increases Bax expression in cancer cell lines   (Citations: 48)
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The NF-κB transcription factor has been shown to inhibit apoptosis in several experimental systems. We therefore investigated whether the expression of the Bax proapoptotic protein could be influenced by NF-κB activity. Increased Bax protein expression was detected in HCT116, OVCAR-3 and MCF7 cells stably expressing a mutated unresponsive IκB-α inhibitory protein that blocks NF-κB activity. Northern blots showed that bax mRNA expression was increased as a consequence of mutated IκB-α expression in HCT116 cells. A careful examination of the human bax gene promoter sequence showed three putative binding sites for NF-κB, and the κB2 site at position -687 could indeed bind NF-κB complexes in vitro. Transient transfection of a bax promoter luciferase construct in HCT116 cells showed that NF-κB proteins could partially inhibit the transactivation of the bax promoter by p53. Mutations or deletions of the κB sites, including κB2, indicated that this NF-κB-dependent inhibitory effect did not require NF-κB DNA-binding, and was thus an indirect effect. However, cotransfection of expression vectors for several known cofactors failed to identify a competition between p53 and NF-κB for a transcription coactivator. Our findings thus demonstrate for the first time that NF-κB regulates, through an indirect pathway, the bax gene expression.
Journal: Oncogene , vol. 20, no. 22, pp. 2805-2813, 2001
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