Academic
Publications
The metabolic fate of doubly stable isotope labelled heat-killed Lactobacillus johnsonii in humans

The metabolic fate of doubly stable isotope labelled heat-killed Lactobacillus johnsonii in humans,10.1038/sj.ejcn.1602716,European Journal of Clinica

The metabolic fate of doubly stable isotope labelled heat-killed Lactobacillus johnsonii in humans   (Citations: 1)
BibTex | RIS | RefWorks Download
Objective:In this study, heat-killed Lactobacillus johnsonii (La1), doubly labelled with 13C and 15N (hk-dlLa1), was used to follow the metabolic fate after oral administration in humans.Design:Experimental study.Setting:University of Rostock, Children's Hospital, Research Laboratory.Subjects:Ten healthy adults aged 23–26 years.Intervention:The subjects received 74.6 mg/kg body weight hk-dlLa1 and 10 g α-D-raffinose together with breakfast. A sample of venous blood was taken after 2 h. Expired air samples were taken over 14 h, whereas urine and faeces were collected over a period of 48 h. 13C- and 15N-enrichments were measured by isotope ratio mass spectrometry. Hydrogen concentrations were measured by electrochemical detection.Results:The orocaecal transit time (OCTT) was reached after 3.4 h. After 2 h, 13C- and 15N-enrichment of fibrinogen amounted to 2 and 25 p.p.m. excess, respectively. The 13CO2-exhalation amounted to 9.2% of the ingested dose. The urinary excretion of 13C and 15N was 2.1 and 10.4% of the ingested dose, respectively, whereas the faecal excretion was 47.9 and 43.7% of the ingested dose, respectively.Conclusions:In comparison to OCTT of 3.4 h, both stable isotopes appear after 30 min in breath and urine, indicating that hk-dlLa1 is rapidly digested in the small bowel before reaching the caecum. This is confirmed by 13C-and 15N-enrichments of blood plasma fractions. The ingestion of hk-dlLa1 led to a 13C- and 15N-excretion of 59.2 and 54.1% of the ingested dose, respectively, of both stable isotopes.
Journal: European Journal of Clinical Nutrition - EUR J CLIN NUTR , vol. 62, no. 2, pp. 197-202, 2008
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by: