Academic
Publications
Potential of faecal RNA in diagnosing colorectal cancer

Potential of faecal RNA in diagnosing colorectal cancer,10.1016/j.canlet.2004.11.005,Cancer Letters,Shung-Haur Yang,Chih-Cheng Chien,Chan-Wei Chen,Shi

Potential of faecal RNA in diagnosing colorectal cancer   (Citations: 5)
BibTex | RIS | RefWorks Download
Early diagnosis for colorectal cancer (CRC) by monitoring of gastrointestinal epithelial cells is a possible direct approach. Although human faeces form a heterogeneous mixture of gastrointestinal mucosal epithelial cells and other materials, we have developed a method to purify total RNA from human stool samples. The gene for faecal cytokeratin 19 (CK19) was highly expressed in stools from patients with metastatic CRC, but not from patients with non-metastatic CRC or from normal individuals. Thus, purified faecal RNA can be used for the detection of differentially expressed genes. This technique may help identifying meaningful faecal RNA markers for the non-invasive screening of patients with CRC.
Journal: Cancer Letters - CANCER LETT , vol. 226, no. 1, pp. 55-63, 2005
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
    • ...Total RNA extraction and reverse transcription reaction Total RNA from these cultured cells was extracted using the Easy Pure Total RNA Mini Kit (Bioman, Taipei, Taiwan) according to the manufacturer’s instructions and fecal RNA was prepared as reportedly previously [16]...

    Chi-Jung Huanget al. A predicted protein, KIAA0247, is a cell cycle modulator in colorectal...

    • ...We previously reported that the upregulation of CK19 in feces predicted the presence of metastasis [20]...
    • ...Fecal samples and colonic tumor specimens Solid fecal samples were collected from the 129 patients prior to any surgical or chemical therapy, as described in our previous report [20]...
    • ...Quantitation of fecal CK19 expression Fecal total RNA and cDNA were obtained as described in our previous reports [20,22]...
    • ...Because of the deregulation of apoptosis in CRC cells, cancer cells in feces are thought to be more stable than other cells sloughed from normal mucosa [23] and could even represent a target for the molecular diagnosis of CRC [24]; therefore, fecal molecules from exfoliated colonic cells are currently being developed as potential markers for colonic neoplasia [20]...
    • ...We reported several fecal molecules that are differentially expressed in CRC patients [21,22], and demonstrated that CK19 expression in feces is possibly correlated with CRC metastasis [20]...

    Chun-Chao Changet al. Clinical meaning of age-related expression of fecal cytokeratin 19 in ...

Sort by: