Adiponectin increases insulin-like growth factor I-induced progesterone and estradiol secretion in human granulosa cells,10.1016/j.fertnstert.2008.09.

• ...Additionally, our team has found in rat [2], chicken [15] and human [16], that physiological levels of recombinant human adiponectin (rh adiponectin, 5 or 10 μg/ml) are able to increase progesterone and/or estradiol secretions in response to insulinlike growth factor-I (IGF-I) in cultured granulosa cells...
• ...Recently, AdipoR1 and AdipoR2 expression has been reported in ovary in human [16], pig [21], rodents [2], chicken [15] and cow [22]...
• ...Expression of adiponectin and its two receptors has already been reported in ovarian structures of several species, including pig [21,40], rat [2], chicken [15,41,42] and human [16]...
• ...Indeed, other studies on human or rat and chicken have observed no [16] or a weak [2,15] mRNA and protein expression of adiponectin in granulosa cells, respectively...
• ...In agreement with results obtained in rat and human granulosa cells [2,16], we observed no effect of adiponectin on basal granulosa cell proliferation in cow...
• ...In rat [2] and human [16], the effect of adiponectin (5 μg/mL) was also studied on granulosa cell proliferation in the presence of IGF-1, but adiponectin treatment had no effect...
• ...This lack of adiponectin effect on basal steroidogenesis is not specific to the bovine species or to the adiponectin concentration used in experiments, since it has been previously observed in rat with 5 μg/mL of adiponectin [2], in chicken with a 10 μg/mL dose [15] and in human with a 5 μg/mL concentration [16]...
• ...Maillard et al. Reproductive Biology and Endocrinology 2010, 8:23 http://www.rbej.com/content/8/1/23 Page 12 of 15 [16], hen [15] and rat [2]...
• ...However, a recent study in human also revealed that adiponectin (5 μg/ mL) did not activate AMPK in granulosa cells [16]...
• ...Adiponectin rapidly and transiently activated ERK1/2 MAPK in bovine granulosa cells, as previously described in porcine, rat, chicken and human granulosa cells [2,15,16,40]...

Virginie Maillard, et al. Effect of adiponectin on bovine granulosa cell steroidogenesis, oocyte...