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Evaluation of endometrial biomarkers for semi-invasive diagnosis of endometriosis

Evaluation of endometrial biomarkers for semi-invasive diagnosis of endometriosis,10.1016/j.fertnstert.2010.06.084,Fertility and Sterility,Cleophas M.

Evaluation of endometrial biomarkers for semi-invasive diagnosis of endometriosis   (Citations: 2)
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Journal: Fertility and Sterility - FERT STERIL , vol. 95, no. 4, pp. 1338-1343.e3, 2011
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    • ...Numerous proteomic [3-10] and mRNA microarray [11-14] studies have demonstrated important biological differences between eutopic endometrium from women with and without endometriosis...
    • ...peaks identified in EM samples in our previous studies [5,6] were not confirmed in the present study...
    • ...In our second study [5], the combination of SELDI-TOF MS ProteinChip technology with bioinformatics allowed us to develop a diagnostic test for minimal-mild endometriosis based on a panel of 4 mass peaks (2 up-regulated: 90.675 kDa and 35.956 kDa and 2 down-regulated: 1.9 kDa and 2.5 kDa) with maximal sensitivity (100%) and specificity (100%)...
    • ...Firstly, the protein extraction method was based on TRIzol (Invitrogen Life Technologies, Carlsbad CA, USA) in the current study and on U9 lysis buffer (Ciphergen Biosystems, Fremont, CA) in our previous studies [5,6]...
    • ...secretory phase (day 16 - 26) in our previous studies [5,6]...

    Amelie Fassbenderet al. TRIzol treatment of secretory phase endometrium allows combined proteo...

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