Serum deprivation induced apoptosis in macrophage is mediated by autocrine secretion of type I IFNs
Apoptosis can be triggered by different forms of cellular stress. We here show that serum deprivation induces the expression
and secretion of type I interferons and results in apoptosis in RAW 264.7 cell in a caspase dependent manner. Administration
of either IFN-α or IFN-β antibody partially inhibits apoptosis while the two antibodies used together totally prevents RAW264.7
cell from apoptosis. GM-CSF, but not M-CSF and IL-3, protects serum deprivation induced apoptosis. Inhibition of JAKs also
prevents macrophages from apoptosis. Activation of MAPKs is not required for serum deprivation induced apoptosis. Our results
are the first to demonstrate that serum deprivation-induced apoptosis acts through autocrine secretion of type I interferons.
, vol. 11, no. 4, pp. 545-554, 2006