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Abnormal HNK-1 expression in the cerebellum of an N-CAM null mouse

Abnormal HNK-1 expression in the cerebellum of an N-CAM null mouse,10.1023/B:NEUR.0000029652.96456.0d,Journal of Neurocytology,Hassan Marzban,Roy V. S

Abnormal HNK-1 expression in the cerebellum of an N-CAM null mouse   (Citations: 20)
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Human natural killer antigen-1 (HNK-1) is a carbohydrate epitope associated with sulfoglucuronylglycolipids and glycoproteins. Biochemical analyses have demonstrated associations between the HNK-1 epitope and isoforms of the neural cell adhesion molecule (N-CAM) family. In the cerebellum, HNK-1 is prominently expressed in Purkinje cell dendrites and Golgi cells. Purkinje cell expression of HNK-1 reveals an array of parasagittal stripes and transverse zones. Interestingly, the parasagittal expression pattern of HNK-1 is different from those reported with several other markers such as zebrin II/aldolase C and the small heat shock protein HSP25. N-CAM null knockout mice were used to explore the possible role of the HNK-1/N-CAM interaction during the topographical organization of the cerebellar cortex. N-CAM null mice have no N-CAM immunoreactivity but otherwise the cerebellum appears morphologically normal. Further, in the N-CAM null HNK-1 immunoreactivity is abolished from Purkinje cell dendrites but is retained on Golgi cells and neurons of the cerebellar nuclei. Despite the absence of N-CAM/HNK-1, parasagittal stripes and transverse zones in the cerebellum as revealed by using zebrin II immunocytochemistry appear normal.
Journal: Journal of Neurocytology - J NEUROCYTOL , vol. 33, no. 1, pp. 117-130, 2004
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    • ... For example, heat shock protein 25 (HSP25) and human natural killer antigen 1 (HNK1) are expressed by subsets of the zebrin II+ population of the CZ and N...

    Richard Hawkeset al. Cerebellar cortical organization: a one-map hypothesis

    • ...Culture supernatants were harvested every 2–3 days and used for immunocytochemistry without further purification (diluted 1:50; see [15, 16])...
    • ...PLCβ4 immunoreactivity in the granular layer is not associated with Golgi cell somata— there is no coexpression with two Golgi cell markers—a glycine transporter 2-EGFP transgene (Fig. 1e) [11] and HNK-1 (Fig. 1f) [15, 16]...
    • ...Differences between P1+−P3+ stripes in lobule IX, despite similar zebrin II expression, as suggested by the differential distribution of UBC subtypes, are echoed in their molecular phenotypes: For example, the midline P1+/P2+ zebrin II stripes are HNK-1 immunonegative, but P3+ is HNK-1+ [16]...

    Seung-Hyuk Chunget al. Phospholipase Cβ4 Expression Identifies a Novel Subset of Unipolar Bru...

    • ...First, anti-neurogranin immunohistochemistry,toidentifythedendriticarborsofGolgicells(Singecet al.,2003),wascombinedwithPurkinjecellstripeantigens,zebrin II/aldolaseC(Brochuetal.,1990;Ahnetal.,1994),HNK-1(Marzban et al., 2004), and phospholipase C4 (Sarna et al., 2006), to explore interneuron cytoarchitecture in the context of cerebellar topography...
    • ...Restriction of Purkinje cell dendrites is not confined to zebrin II/ boundaries: a similar pattern of Golgi cell restriction was also found both at boundaries revealed by HNK-1 expression in ventral lobule IX and lobule X [the nodular zone (Eisenman and Hawkes, 1993; Marzban et al., 2004)], where all Purkinje cells are zebrin II (Fig. 3E), and at boundaries of expression of PLC4 (see Fig. 5A,B) [unsurprisingly, because PLC4 expression is ...

    R. V. Sillitoeet al. Golgi Cell Dendrites Are Restricted by Purkinje Cell Stripe Boundaries...

    • ...Peroxidase immunohistochemistry was carried out on free-floating sections as described previously (41)...
    • ...Cerebellar sections for fluorescent immunohistochemistry were processed as described previously (41)...
    • ... expression pattern to that of zebrin II – for example, PLCß4 (39) and Ebf2 (47); (iii) markers that identify subsets within the zebrin II populations – for example, the small heat shock protein (HSP) 25 is constitutively expressed in stripes solely in the (uniformly zebrin II-immunopositive) CZ, NZ and flocculus (8), the human natural killer cell antigen HNK-1 is expressed in zebrin II-immunopositive stripes in the PZ but not elsewhere ...

    Seung-Hyuk Chunget al. Compartmentation of gaba b receptor2 expression in the mouse cerebella...

    • ...For example, comparisons between zebrin II and the glycoprotein epitope HNK1 reveal that these two antigens are largely co-localized (26) but are also expressed in discrete Purkinje cell populations in several lobules (27)...

    Matt Laroucheet al. From clusters to stripes: The developmental origins of adult cerebella...

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