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High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts

High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts,10.1007/s11248-008-9209-0,Transgenic Research,M. Nadai,J.

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High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts (Citations: 5)


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We have produced human alpha1-antitrypsin (A1AT), a major therapeutic protein, in genetically engineered tobacco plastids. Four different expression vectors have been evaluated which encode A1AT under the control of various 5′ and 3′ plastid expression elements. The use of heterologous promoter and terminator sequences derived from the corn and soybean plastid genomes leads to simpler and predictable recombinant genome patterns, avoiding unwanted recombination products between introduced and resident tobacco sequences. High level expression of unglycosylated A1AT, representing up to 2% of total soluble proteins, has been measured in leaves of transgenic tobacco lines. Some heterogeneity in the recombinant A1AT is detected after 2D protein separation, but the chloroplast-made protease inhibitors are fully active and bind to porcine pancreatic elastase.

Journal: Transgenic Research - TRANSGENIC RES , vol. 18, no. 2, pp. 173-183, 2009

DOI: 10.1007/s11248-008-9209-0

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- ...) was designed to minimize the use of endogenous tobacco regulatory elements, therefore eliminating the possibility of foreign gene loss through deleterious homologous recombination between the duplicated sequences in the transformed plastome ...
Igor Kolotilin, et al. Production of a Subunit Vaccine Candidate against Porcine Post-Weaning...

Isoelectric focusing as a tool for the investigation of post-translational processing and chemical modifications of proteins (Citations: 18)

Journal: Journal of Chromatography A - J CHROMATOGR A , vol. 705, no. 1, pp. 67-87, 1995

High-level secretion of human α 1-antitrypsin from Saccharomyces cerevisiae using inulinase signal sequence (Citations: 11)

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Journal: Journal of Biotechnology - J BIOTECHNOL , vol. 48, no. 1, pp. 15-24, 1996

Purification and characterization of α 1-antitrypsin secreted by recombinant yeast Saccharomyces diastaticus (Citations: 13)

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Journal: Journal of Biotechnology - J BIOTECHNOL , vol. 42, no. 3, pp. 191-195, 1995

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Citations (5)

Production of a Subunit Vaccine Candidate against Porcine Post-Weaning Diarrhea in High-Biomass Transplastomic Tobacco

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Journal: PLOS One , vol. 7, no. 8, 2012

Expression and characterization of antimicrobial peptides Retrocyclin-101 and Protegrin-1 in chloroplasts to control viral and bacterial infections: Plant-made antimicrobial peptides

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Journal: Plant Biotechnology Journal - PLANT BIOTECHNOL J , pp. no-no, 2010

Keywords (7)

High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts,10.1007/s11248-008-9209-0,Transgenic Research,M. Nadai,J.

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Igor Kolotilin, et al. Production of a Subunit Vaccine Candidate against Porcine Post-Weaning...

References (60)

Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis (Citations: 52)

Production of functional human α 1 -antitrypsin by plant cell culture (Citations: 59)