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Noninvasive Measurement of Airway Responsiveness in Allergic Mice Using Barometric Plethysmography

Noninvasive Measurement of Airway Responsiveness in Allergic Mice Using Barometric Plethysmography,E. HAMELMANN,J. SCHWARZE,K. TAKEDA,A. OSHIBA,G. L.

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Noninvasive Measurement of Airway Responsiveness in Allergic Mice Using Barometric Plethysmography   (Citations: 497)
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To study the mechanisms and kinetics underlying the development of increased airway responsive- ness (AR) after allergic sensitization, animal models have been invaluable. Using barometric whole- body plethysmography and increases in enhanced pause (Penh) as an index of airway obstruction, we measured responses to inhaled methacholine in conscious, unrestrained mice after sensitization and airway challenge with ovalbumin (OVA). Sensitized and challenged animals had significantly in- creased AR to aerosolized methacholine compared with control animals. AR measured as Penh was associated with increased IgE production and eosinophil lung infiltration. In a separate approach we confirmed the involvement of the lower airways in the response to aerosolized methacholine using tracheotomized mice. Increases in Penh values after methacholine challenge were also correlated with increased intrapleural pressure, measured via an esophageal tube. Lastly, mice demonstrating AR using a noninvasive technique also demonstrated increased pulmonary resistance responses to aerosolized methacholine when measured using an invasive technique the following day in the same animals. The increases in Penh values were inhibited by pretreatment of the mice with a b 2 -agonist. These data indicate that measurement of AR to inhaled methacholine by barometric whole-body plethysmography is a valid indicator of airway hyperresponsiveness after allergic sensitization in mice. The measurement of AR in unrestrained, conscious animals provides new opportunities to eval- uate the mechanisms and kinetics underlying the development and maintenance of airway hyperre- sponsiveness and to assess various therapeutic interventions. Hamelmann E, Schwarze J, Takeda K, Oshiba A, Larsen GL, Irvin CG, Gelfand EW. Noninvasive measurement of airway responsive- ness in allergic mice using barometric plethysmography. AM J RESPIR CRIT CARE MED 1997;156:766-775.
Published in 1997.
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